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Applied and Environmental Microbiology, March 2003, p. 1861-1865, Vol. 69, No. 3
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.3.1861-1865.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Heterologous Expression of the Saccharomyces cerevisiae PGU1 Gene in Schizosaccharomyces pombe Yields an Enzyme with More Desirable Properties for the Food Industry{dagger}

C. Sieiro, M. Poza, M. Vilanova, and T. G. Villa*

Department of Microbiology, Faculty of Pharmacy, University of Santiago de Compostela, Santiago de Compostela, Spain

Received 9 July 2002/ Accepted 28 December 2002

The Saccharomyces cerevisiae PGU1 gene was successfully expressed in Schizosaccharomyces pombe. The optimum pH and temperature for the recombinant enzyme were 5 and 40°C, respectively, these being around 0.5 U higher and 5°C lower than those shown by the native enzyme. The Km value was about fourfold higher than that of the S. cerevisiae enzyme. The recombinant endopolygalacturonase was more efficient in reducing the viscosity of polygalacturonic acid and was also more stable at different pHs and temperatures than the native enzyme.

* Corresponding author. Mailing address: Departamento de Microbiología, Facultad de Farmacia, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain. Phone: 34 981 592490. Fax: 34 981 592490. E-mail: mpvilla{at}usc.es.

{dagger} This paper is dedicated to the memory of Herman Jan Phaff, a pioneer in the field of yeast polygalacturonases as early as the 1950s.

Applied and Environmental Microbiology, March 2003, p. 1861-1865, Vol. 69, No. 3
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.3.1861-1865.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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