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Applied and Environmental Microbiology, April 2003, p. 1884-1889, Vol. 69, No. 4
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.4.1884-1889.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification of Streptomyces coelicolor Proteins That Are Differentially Expressed in the Presence of Plant Material

P. Langlois,1 S. Bourassa,2 G. G. Poirier,2 and C. Beaulieu1*

Centre d'Étude et de Valorisation de la Diversité Microbienne, Département de Biologie, Université de Sherbrooke, Sherbrooke, Québec, Canada J1K 2R1,1 Centre Protéomique de l'Est du Québec, Centre Hospitalier de l'Université Laval, Sainte-Foy, Québec, Canada G1V 4G22

Received 30 July 2002/ Accepted 11 November 2002

Streptomyces coelicolor and Lemna minor were used as a model to study the modulation of bacterial gene expression during plant-streptomycete interactions. S. coelicolor was grown in minimal medium with and without L. minor fronds. Bacterial proteomes were analyzed by two-dimensional gel electrophoresis, and a comparison of the two culture conditions resulted in identification of 31 proteins that were induced or repressed by the presence of plant material. One-half of these proteins were identified by peptide mass fingerprinting by using matrix-assisted laser desorption ionization-time of flight mass spectrometry. The induced proteins were involved in energetic metabolism (glycolysis, pentose phosphate pathway, oxidative phosphorylation), protein synthesis, degradation of amino acids, alkenes, or cellulose, tellurite resistance, and growth under general physiological or oxidative stress conditions. The repressed proteins were proteins synthesized under starvation stress conditions. These results suggest that root exudates provide additional carbon sources to the bacteria and that physiological adaptations are required for efficient bacterial growth in the presence of plants.

* Corresponding author. Mailing address: Département de Biologie, Université de Sherbrooke, Sherbrooke, Québec, Canada J1K 2R1. Phone: (819) 821-8000, ext. 2997. Fax: (819) 821-8049. E-mail: Carole.Beaulieu{at}USherbrooke.ca.

Applied and Environmental Microbiology, April 2003, p. 1884-1889, Vol. 69, No. 4
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.4.1884-1889.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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