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Applied and Environmental Microbiology, May 2003, p. 2498-2504, Vol. 69, No. 5
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.5.2498-2504.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Cloning and Sequencing of a Poly(DL-Lactic Acid) Depolymerase Gene from Paenibacillus amylolyticus Strain TB-13 and Its Functional Expression in Escherichia coli

Yukie Akutsu-Shigeno,1,2 Teerawat Teeraphatpornchai,1 Kamonluck Teamtisong,3 Nobuhiko Nomura,1 Hiroo Uchiyama,1 Tadaatsu Nakahara,1 and Toshiaki Nakajima-Kambe1,2*

PRESTO, Japan Science and Technology Corporation,1 Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan,2 Institute of Agricultural Technology, School of Biotechnology, Suranaree University of Technology, Nakhon-Ratchasima, Thailand 300003

Received 4 November 2002/ Accepted 24 January 2003

The gene encoding a poly(DL-lactic acid) (PLA) depolymerase from Paenibacillus amylolyticus strain TB-13 was cloned and overexpressed in Escherichia coli. The purified recombinant PLA depolymerase, PlaA, exhibited degradation activities toward various biodegradable polyesters, such as poly(butylene succinate), poly(butylene succinate-co-adipate), poly(ethylene succinate), and poly({epsilon}-caprolactone), as well as PLA. The monomeric lactic acid was detected as the degradation product of PLA. The substrate specificity toward triglycerides and p-nitrophenyl esters indicated that PlaA is a type of lipase. The gene encoded 201 amino acid residues, including the conserved pentapeptide Ala-His-Ser-Met-Gly, present in the lipases of mesophilic Bacillus species. The identity of the amino acid sequence of PlaA with Bacillus lipases was no more than 45 to 50%, and some of its properties were different from those of these lipases.

* Corresponding author. Mailing address: Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan. Phone: 81-298-53-4927. Fax: 81-298-53-4605. E-mail: toshi{at}sakura.cc.tsukuba.ac.jp.

Applied and Environmental Microbiology, May 2003, p. 2498-2504, Vol. 69, No. 5
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.5.2498-2504.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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