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Applied and Environmental Microbiology, May 2003, p. 2657-2663, Vol. 69, No. 5
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.5.2657-2663.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Novel Eukaryotic Lineages Inferred from Small-Subunit rRNA Analyses of Oxygen-Depleted Marine Environments{dagger}

Thorsten Stoeck* and Slava Epstein

Marine Science Center, Northeastern University, Nahant, Massachusetts 01908

Received 20 November 2002/ Accepted 6 February 2003

Microeukaryotes in oxygen-depleted environments are among the most diverse, as well as the least studied, organisms. We conducted a cultivation-independent, small-subunit (SSU) rRNA-based survey of microeukaryotes in suboxic waters and anoxic sediments in the great Sippewisset salt marsh, Cape Cod, Mass. We generated two clone libraries and analyzed approximately 300 clones, which contained a large diversity of microeukaryotic SSU rRNA signatures. Only a few of these signatures were closely related (sequence similarity of >97%) to the sequences reported earlier. The bulk of our sequences represented deep novel branches within green algae, fungi, cercozoa, stramenopiles, alveolates, euglenozoa and unclassified flagellates. In addition, a significant number of detected rRNA sequences exhibited no affiliation to known organisms and sequences and thus represent novel lineages of the highest taxonomical order, most of them branching off the base of the global phylogenetic tree. This suggests that oxygen-depleted environments harbor diverse communities of novel organisms, which may provide an interesting window into the early evolution of eukaryotes.

* Corresponding author. Mailing address: Northeastern University, Marine Science Center, East Point, Nahant, MA 01908. Phone: (781) 581-7370. Fax: (781) 581-6076. E-mail: thorsten.stoeck{at}web.de.

{dagger} This is contribution no. 245 of the Marine Science Center of Northeastern University.

Applied and Environmental Microbiology, May 2003, p. 2657-2663, Vol. 69, No. 5
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.5.2657-2663.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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