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Applied and Environmental Microbiology, May 2003, p. 2748-2754, Vol. 69, No. 5
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.5.2748-2754.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Homologous npdGI Genes in 2,4-Dinitrophenol- and 4-Nitrophenol-Degrading Rhodococcus spp.

Gesche Heiss,^1* Natalie Trachtmann,¹ Yoshikatsu Abe,² Masahiro Takeo,^1,2 and Hans-Joachim Knackmuss¹

Institute of Microbiology, University of Stuttgart, 70550 Stuttgart, Germany,¹ Department of Applied Chemistry, Himeji Institute of Technology, Himeji, Hyogo, Japan²

Received 26 September 2002/ Accepted 13 February 2003

Rhodococcus (opacus) erythropolis HL PM-1 grows on 2,4,6-trinitrophenol or 2,4-dinitrophenol (2,4-DNP) as a sole nitrogen source. The NADPH-dependent F₄₂₀ reductase (NDFR; encoded by npdG) and the hydride transferase II (HTII; encoded by npdI) of the strain were previously shown to convert both nitrophenols to their respective hydride Meisenheimer complexes. In the present study, npdG and npdI were amplified from six 2,4-DNP degrading Rhodococcus spp. The genes showed sequence similarities of 86 to 99% to the respective npd genes of strain HL PM-1. Heterologous expression of the npdG and npdI genes showed that they were involved in 2,4-DNP degradation. Sequence analyses of both the NDFRs and the HTIIs revealed conserved domains which may be involved in binding of NADPH or F₄₂₀. Phylogenetic analyses of the NDFRs showed that they represent a new group in the family of F₄₂₀-dependent NADPH reductases. Phylogenetic analyses of the HTIIs revealed that they form an additional group in the family of F₄₂₀-dependent glucose-6-phosphate dehydrogenases and F₄₂₀-dependent N⁵,N¹⁰-methylenetetrahydromethanopterin reductases. Thus, the NDFRs and the HTIIs may each represent a novel group of F₄₂₀-dependent enzymes involved in catabolism.

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* Corresponding author. Mailing address: Institute of Microbiology, University of Stuttgart, Allmandring 31, 70550 Stuttgart, Germany. Phone: 49-711-685-5489. Fax: 49-711-685-5725. E-mail: gesche.heiss{at}po.uni-stuttgart.de.

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Applied and Environmental Microbiology, May 2003, p. 2748-2754, Vol. 69, No. 5
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.5.2748-2754.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

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