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Applied and Environmental Microbiology, June 2003, p. 3658-3662, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3658-3662.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Phylogeny and Characterization of Three nifH-Homologous Genes from Paenibacillus azotofixans

Quok-Cheong Choo, Mohd-Razip Samian, and Nazalan Najimudin*

School of Biological Sciences, Universiti Sains Malaysia, 11800 Pulau Pinang, Malaysia

Received 19 November 2002/ Accepted 21 March 2003

In this paper, we report the cloning and characterization of three Paenibacillus azotofixans DNA regions containing genes involved in nitrogen fixation. Sequencing analysis revealed the presence of nifB1H1D1K1 gene organization in the 4,607-bp SacI DNA fragment. This is the first report of linkage of a nifB open reading frame upstream of the structural nif genes. The second (nifB2H2) and third (nifH3) nif homologues are confined within the 6,350-bp HindIII and 2,840-bp EcoRI DNA fragments, respectively. Phylogenetic analysis demonstrated that NifH1 and NifH2 form a monophyletic group among cyanobacterial NifH proteins. NifH3, on the other hand, clusters among NifH proteins of the highly divergent methanogenic archaea.

* Corresponding author. Mailing address: School of Biological Sciences, Universiti Sains Malaysia, 11800 Pulau Pinang, Malaysia. Phone: 60-4-8603181. Fax: 60-4-6565125. E-mail: nazalan{at}usm.my.

Applied and Environmental Microbiology, June 2003, p. 3658-3662, Vol. 69, No. 6
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.6.3658-3662.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kasap, M., Chen, J.-S. (2005). Clostridium pasteurianum W5 synthesizes two NifH-related polypeptides under nitrogen-fixing conditions. Microbiology 151: 2353-2362 [Abstract] [Full Text]  


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