This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Williams, J.
Right arrow Articles by Cooper, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Williams, J.
Right arrow Articles by Cooper, R. M.
Agricola
Right arrow Articles by Williams, J.
Right arrow Articles by Cooper, R. M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, July 2003, p. 4190-4191, Vol. 69, No. 7
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.7.4190-4191.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

A Selective Medium for Quantitative Reisolation of Trichoderma harzianum from Agaricus bisporus Compost

Josie Williams,1 John M. Clarkson,1,{dagger} Peter R. Mills,2 and Richard M. Cooper1*

Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY,1 Department of Microbial Biotechnology, Horticultural Research International, Wellesbourne, Warwick CV35 9EF, United Kingdom2

Received 14 April 2003/ Accepted 17 April 2003

We adapted a selective medium, previously developed for reisolation of Trichoderma spp. from soil, for quantitative determination of growth of T. harzianum from commercial Agaricus bisporus composts. This medium enables comparisons of aggressive (sensu inhibition of A. bisporus yield) with nonaggressive T. harzianum groups. The resulting medium contains the antimicrobials chloramphenicol, streptomycin, quintozene, and propamocarb and was highly selective, allowing the recovery of T. harzianum, as viable conidia and hyphal fragments, in compact colonies with the absence of visible microbial contaminants.

* Corresponding author. Mailing address: Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom. Phone: 44 0 1225 383051. Fax: 44 0 1225 826779. Email: bssrmc{at}bath.ac.uk.

{dagger} Present address: Molecular Sensing plc, Melksham, Wiltshire SN12 8HL, United Kingdom.

Applied and Environmental Microbiology, July 2003, p. 4190-4191, Vol. 69, No. 7
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.7.4190-4191.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»