AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bertaux, J.
Right arrow Articles by Frey-Klett, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bertaux, J.
Right arrow Articles by Frey-Klett, P.
Agricola
Right arrow Articles by Bertaux, J.
Right arrow Articles by Frey-Klett, P.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, July 2003, p. 4243-4248, Vol. 69, No. 7
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.7.4243-4248.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

In Situ Identification of Intracellular Bacteria Related to Paenibacillus spp. in the Mycelium of the Ectomycorrhizal Fungus Laccaria bicolor S238N

J. Bertaux,1 M. Schmid,2 N. Chemidlin Prevost-Boure,1 J. L. Churin,1 A. Hartmann,2 J. Garbaye,1 and P. Frey-Klett1*

Institut National de la Recherche Agronomique, UMR "Interactions Arbres/Micro-organismes," Centre de Nancy, 54280 Champenoux, France,1 Department of Rhizosphere Biology, Institute of Soil Ecology, GSF-National Research Center for Environment and Health, D-85764 Neuherberg/Munich, Germany2

Received 14 February 2003/ Accepted 30 April 2003

Bacterial proliferations have recurrently been observed for the past 15 years in fermentor cultures of the ectomycorrhizal fungus Laccaria bicolor S238N, suggesting the presence of cryptic bacteria in the collection culture of this fungus. In this study, intracellular bacteria were detected by fluorescence in situ hybridization in combination with confocal laser scanning microscopy in several collection subcultures of L. bicolor S238N. They were small (0.5 µm in diameter), rare, and heterogeneously distributed in the mycelium and were identified as Paenibacillus spp. by using a 16S rRNA-directed oligonucleotide probe initially designed for bacteria isolated from a fermentor culture of L. bicolor S238N.

* Corresponding author. Mailing address: Institut National de la Recherche Agronomique, UMR "Interactions Arbres/Micro-organismes," Centre de Nancy, 54280 Champenoux, France. Phone: 33 383394149. Fax: 33 383394069. E-mail: klett{at}nancy.inra.fr.

Applied and Environmental Microbiology, July 2003, p. 4243-4248, Vol. 69, No. 7
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.7.4243-4248.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2003 by the American Society for Microbiology. All rights reserved.