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Applied and Environmental Microbiology, July 2003, p. 4272-4273, Vol. 69, No. 7
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.7.4272-4273.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Rapid Staining and Enumeration of Small Numbers of Total Bacteria in Water by Solid-Phase Laser Cytometry

Susan C. Broadaway, Stephanie A. Barton, and Barry H. Pyle*

Microbiology Department, Montana State University, Bozeman, Montana 59717

Received 3 June 2002/ Accepted 23 April 2003

The nucleic acid stain SYBR Green I was evaluated for use with solid-phase laser cytometry to obtain total bacterial cell counts from several water sources with small bacterial numbers. Results were obtained within 30 min and exceeded or equaled counts on R2A agar plates incubated for 14 days at room temperature.

* Corresponding author. Mailing address: Microbiology Department, Montana State University—Bozeman, Bozeman, MT 59717. Phone: (406) 994-3041. Fax: (406) 994-4926. E-mail: barryp{at}montana.edu.

Applied and Environmental Microbiology, July 2003, p. 4272-4273, Vol. 69, No. 7
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.7.4272-4273.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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