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Applied and Environmental Microbiology, July 2003, p. 4274-4277, Vol. 69, No. 7
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.7.4274-4277.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Design and Application of a New Cryptic-Plasmid-Based Shuttle Vector for Magnetospirillum magneticum

Yoshiko Okamura,1 Haruko Takeyama,1 Takumi Sekine,1 Toshifumi Sakaguchi,1 Aris Tri Wahyudi,1 Rika Sato,2 Shinji Kamiya,2 and Tadashi Matsunaga1*

Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, Tokyo,1 TDK Akita Laboratory Corporation, Akita, Japan2

Received 10 October 2002/ Accepted 23 April 2003

A 3.7-kb cryptic plasmid designated pMGT was found in Magnetospirillum magneticum MGT-1. It was characterized and used for the development of an improved expression system in strain AMB-1 through the construction of a shuttle vector, pUMG. An electroporation method for magnetic bacteria that uses the cryptic plasmid was also developed.

* Corresponding author. Mailing address: Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Koganei, Tokyo 184-8588, Japan. Phone: 81-423-88-7020. Fax: 81-423-85-7713. E-mail: tmatsuna{at}cc.tuat.ac.jp.

Applied and Environmental Microbiology, July 2003, p. 4274-4277, Vol. 69, No. 7
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.7.4274-4277.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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  • Maeda, Y., Yoshino, T., Takahashi, M., Ginya, H., Asahina, J., Tajima, H., Matsunaga, T. (2008). Noncovalent Immobilization of Streptavidin on In Vitro- and In Vivo-Biotinylated Bacterial Magnetic Particles. Appl. Environ. Microbiol. 74: 5139-5145 [Abstract] [Full Text]  
  • Tanaka, M., Nakata, Y., Mori, T., Okamura, Y., Miyasaka, H., Takeyama, H., Matsunaga, T. (2008). Development of a Cell Surface Display System in a Magnetotactic Bacterium, "Magnetospirillum magneticum" AMB-1. Appl. Environ. Microbiol. 74: 3342-3348 [Abstract] [Full Text]  
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