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Applied and Environmental Microbiology, July 2003, p. 4291-4295, Vol. 69, No. 7
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.7.4291-4295.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Environmental Isolates of Aeromonas spp. Harboring the cagA-Like Gene of Helicobacter pylori

Simanti Datta,1 Asis Khan,1 Ranjan K. Nandy,2 Motiur Rehman,3 Sutapa Sinha,1 Santanu Chattopadhyay,1 Suresh C. Das,4 and G. Balakrish Nair1,3*

National Institute of Cholera and Enteric Diseases,1 ICMR Virus Unit, Calcutta, Beliaghata, Calcutta 700 010,2 Indian Veterinary Research Institute, Belgachia, Calcutta 700 037, India,4 International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh3

Received 17 September 2002/ Accepted 28 March 2003

We investigated the presence of cagA-like gene of Helicobacter pylori in environmental isolates of Aeromonas spp. from different water samples of Calcutta, India, by colony hybridization using a cagA-specific DNA probe and by PCR with cagA-specific primers. Nucleotide sequencing of five PCR products revealed 97 to 98% homology to canonical cagA of H. pylori 26695 as well as to four clinical H. pylori strains from Calcutta. The cagA-like gene of the environmental isolates was unstable in laboratory conditions and tended to be lost upon subculturing.

* Corresponding author. Mailing address: Laboratory Sciences Division, International Centre for Diarrheal Disease Research, Bangladesh (ICDDR, B), Mohakhali, Dhaka-1212, Bangladesh. Phone: 880-2-9886484. Fax: 880-2-8823116. E-mail: gbnair{at}icddrb.org.

Applied and Environmental Microbiology, July 2003, p. 4291-4295, Vol. 69, No. 7
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.7.4291-4295.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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