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Applied and Environmental Microbiology, July 2003, p. 4308-4311, Vol. 69, No. 7
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.7.4308-4311.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Use of Root Organ Cultures To Investigate the Interaction between Glomus intraradices and Pratylenchus coffeae

Annemie Elsen,1* Stephane Declerck,2 and Dirk De Waele1

Laboratory of Tropical Crop Improvement, Katholieke Universiteit Leuven, 3001 Leuven,1 Mycothèque de l'Université catholique de Louvain, Unité de Microbiologie, Université catholique de Louvain, 1348 Louvain-la-Neuve, Belgium2

Received 31 October 2002/ Accepted 28 March 2003

The interaction between Glomus intraradices and Pratylenchus coffeae on transformed carrot roots was studied in root organ culture. G. intraradices provided the roots with increased protection against P. coffeae by suppressing nematode reproduction in the roots. The internal and external mycorrhizal development was not influenced by the presence of the nematodes.

* Corresponding author. Mailing address: Laboratory of Tropical Crop Improvement, Katholieke Universiteit Leuven, Kasteelpark Arenberg 13, 3001 Leuven, Belgium. Phone: 32 16 329603. Fax: 32 16 321993. E-mail: annemie.elsen{at}agr.kuleuven.ac.be.

Applied and Environmental Microbiology, July 2003, p. 4308-4311, Vol. 69, No. 7
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.7.4308-4311.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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