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Applied and Environmental Microbiology, August 2003, p. 4611-4617, Vol. 69, No. 8
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.8.4611-4617.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Curvularia Haloperoxidase: Antimicrobial Activity and Potential Application as a Surface Disinfectant

Eva H. Hansen,1,2* Line Albertsen,2 Thomas Schäfer,1 Charlotte Johansen,1,{dagger} Jens C. Frisvad,3 Søren Molin,4 and Lone Gram2

Novozymes A/S, DK-2880 Bagsværd,1 Department of Seafood Research, Danish Institute for Fisheries Research at the Technical University of Denmark,2 Section of Food Biotechnology and Mycology,3 Section of Molecular Microbiology, BioCentrum, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark4

Received 16 September 2002/ Accepted 23 May 2003

A presumed antimicrobial enzyme system, the Curvularia haloperoxidase system, was examined with the aim of evaluating its potential as a sanitizing agent. In the presence of hydrogen peroxide, Curvularia haloperoxidase facilitates the oxidation of halides, such as chloride, bromide, and iodide, to antimicrobial compounds. The Curvularia haloperoxidase system caused several-log-unit reductions in counts of bacteria (Pseudomonas spp., Escherichia coli, Serratia marcescens, Aeromonas salmonicida, Shewanella putrefaciens, Staphylococcus epidermidis, and Listeria monocytogenes), yeasts (Candida sp. and Rhodotorula sp.), and filamentous fungi (Aspergillus niger, Aspergillus tubigensis, Aspergillus versicolor, Fusarium oxysporum, Penicillium chrysogenum, and Penicillium paxilli) cultured in suspension. Also, bacteria adhering to the surfaces of contact lenses were killed. The numbers of S. marcescens and S. epidermidis cells adhering to contact lenses were reduced from 4.0 and 4.9 log CFU to 1.2 and 2.7 log CFU, respectively, after treatment with the Curvularia haloperoxidase system. The killing effect of the Curvularia haloperoxidase system was rapid, and 106 CFU of E. coli cells/ml were eliminated within 10 min of treatment. Furthermore, the antimicrobial effect was short lived, causing no antibacterial effect against E. coli 10 min after the system was mixed. Bovine serum albumin (1%) and alginate (1%) inhibited the antimicrobial activity of the Curvularia haloperoxidase system, whereas glucose and Tween 20 did not affect its activity. In conclusion, the Curvularia haloperoxidase system is an effective sanitizing system and has the potential for a vast range of applications, for instance, for disinfection of contact lenses or medical devices.

* Corresponding author. Mailing address: Danish Institute for Fisheries Research, Department of Seafood Research, Søltofts Plads, c/o Technical University of Denmark, Bldg. 221, DK-2800 Kgs. Lyngby, Denmark. Phone 45 45 25 49 07. Fax: 45 45 88 47 74. E-mail: ehh{at}dfu.min.dk.

{dagger} Present address: Pantheco A/S, DK-2970 Hørsholm, Denmark.

Applied and Environmental Microbiology, August 2003, p. 4611-4617, Vol. 69, No. 8
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.8.4611-4617.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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