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Applied and Environmental Microbiology, September 2003, p. 5670-5672, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5670-5672.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Toward an International Standard for PCR-Based Detection of Food-Borne Thermotolerant Campylobacters: Validation in a Multicenter Collaborative Trial

P. S. Lübeck,1 N. Cook,2 M. Wagner,3 P. Fach,4 and J. Hoorfar1*

Danish Veterinary Institute, DK-1790 Copenhagen V, Denmark,1 DEFRA Central Science Laboratory, Sand Hutton, YO41 1LZ York, United Kingdom,2 Institute for Milk Hygiene and Milk Technology, Vienna, Austria,3 Agence Française de Sécurité Sanitaire des Aliments, Paris, France4

Received 29 January 2003/ Accepted 23 June 2003

As part of a European research project, the performance of a PCR assay to detect food-borne thermotolerant campylobacters (Campylobacter jejuni, C. coli, and C. lari) was evaluated through an international collaborative trial involving 12 participating laboratories. DNA from 10 target and 8 nontarget strains was tested, and the results were reported as the presence of a positive signal after gel electrophoresis. The overall inclusivity (sensitivity) was 93.7%, and the exclusivity (specificity) was 100%. The results indicate that the assay can become an international standard and can be confidently applied in microbiological laboratories.

* Corresponding author. Mailing address: Danish Veterinary Institute, 27 Bülowsvej, DK-1790 Copenhagen V, Denmark. Phone: 45-35 300 251. Fax: 45-35 300 120. E-mail: jho{at}vetinst.dk.

Applied and Environmental Microbiology, September 2003, p. 5670-5672, Vol. 69, No. 9
0099-2240/03/$08.00+0     DOI: 10.1128/AEM.69.9.5670-5672.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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