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Applied and Environmental Microbiology, January 2004, p. 597-598, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.597-598.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Quantum Dots as a Novel Immunofluorescent Detection System for Cryptosporidium parvum and Giardia lamblia

Liang Zhu,1,2 Simon Ang,3 and Wen-Tso Liu1*

Departments of Civil Engineering,1 Mechanical Engineering, National University of Singapore, Singapore,2 Department of Electrical Engineering and Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, Arkansas3

Received 25 July 2003/ Accepted 30 September 2003

Semiconductor quantum dot-conjugated antibodies were successfully developed to label Cryptosporidium parvum and Giardia lamblia. This novel fluorescence system exhibited superior photostability, gave 1.5- to 9-fold-higher signal-to-noise ratios than traditional organic dyes in detecting C. parvum, and allowed dual-color detection for C. parvum and G. lamblia.

* Corresponding author. Mailing address: Department of Civil Engineering, National University of Singapore, Blk E1A, #07-03, Engineering Dr. 2, Singapore 117576, Republic of Singapore. Phone: 65-68741315. Fax: 65-67791635. E-mail: cveliuwt{at}nus.edu.sg.

Applied and Environmental Microbiology, January 2004, p. 597-598, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.597-598.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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