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Applied and Environmental Microbiology, January 2004, p. 607-609, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.607-609.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

A Complete Library of Amino Acid Alterations at N304 in Streptomyces clavuligerus Deacetoxycephalosporin C Synthase Elucidates the Basis for Enhanced Penicillin Analogue Conversion

Hong Soon Chin,1,2 Kian Sim Goo,1 and T. S. Sim1*

Department of Microbiology, Faculty of Medicine,1 Tropical Marine Science Institute, National University of Singapore, Singapore2

Received 18 June 2003/ Accepted 16 October 2003

N304 of Streptomyces clavuligerus deacetoxycephalosporin C synthase was mutagenized to alter its catalytic ability. Given that N304A, N304K, N304L, and N304R mutant enzymes exhibited significant improvements in penicillin analogue conversions, we advocate that replacement of N304 with residues with aliphatic or basic side chains is preferable for engineering of a hypercatalytic enzyme.

* Corresponding author. Mailing address: Department of Microbiology, Faculty of Medicine, National University of Singapore, 5 Science Dr. 2, Singapore 117597, Singapore. Phone: (65) 68743280. Fax: (65) 67766872. E-mail: micsimts{at}nus.edu.sg.

Applied and Environmental Microbiology, January 2004, p. 607-609, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.607-609.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Goo, K. S., Chua, C. S., Sim, T.-S. (2008). Relevant Double Mutations in Bioengineered Streptomyces clavuligerus Deacetoxycephalosporin C Synthase Result in Higher Binding Specificities Which Improve Penicillin Bioconversion. Appl. Environ. Microbiol. 74: 1167-1175 [Abstract] [Full Text]  


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