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Applied and Environmental Microbiology, January 2004, p. 610-612, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.610-612.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Simple and Reliable Method To Precipitate Proteins from Bacterial Culture Supernatant

Randolph B. Caldwell{dagger} and Claus T. Lattemann*

CREATOGEN AG, D-86156 Augsburg, Germany

Received 21 February 2003/ Accepted 4 August 2003

A simple and reliable method for precipitating protein from bacterial culture supernatants based on a pyrogallol red-molybdate-methanol (PRMM) protocol has been developed and applied for the analysis of proteins secreted by a bacterial type III secretion system. PRMM-based precipitation has been shown to be more efficient and robust than are conventional protocols.

* Corresponding author. Present address: Aventis Pharma Deutschland GmbH, Industriepark Höchst, Biocenter H780, 65926 Frankfurt am Main, Germany. Phone: 49-69-30524795. Fax: 49-69-30516371. E-mail: claus.lattemann{at}aventis.com.

{dagger} Present address: GSF, Institut für Molekulare Strahlenbiologie, D-85764 Neuherberg, Germany.

Applied and Environmental Microbiology, January 2004, p. 610-612, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.610-612.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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