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 Previous Article

Applied and Environmental Microbiology, January 2004, p. 642-646, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.642-646.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Neonatal-Mouse Infectivity of Intact Cryptosporidium parvum Oocysts Isolated after Optimized In Vitro Excystation

L. Hou,1 X. Li,1 L. Dunbar,1 R. Moeller,2 B. Palermo,1 and E. R. Atwill1*

Veterinary Medicine Teaching and Research Center, School of Veterinary Medicine, University of California—Davis,1 Tulare Branch, California Animal Health and Food Safety Laboratory, Tulare, California 932742

Received 8 September 2003/ Accepted 22 October 2003

We reexamined the finding of Neumann et al. (10) that intact Cryptosporidium parvum oocysts obtained after in vitro excystation were infectious for neonatal CD-1 mice. We used both established excystation protocols and our own protocol that maximized excystation (2). Although intact oocysts isolated after any of three protocols were infectious for neonatal CD-1 mice, the infectivity of intact oocysts isolated with our optimized excystation protocol was significantly lower than the infectivity of intact oocysts isolated after established protocols or from fresh oocysts. Excystation should not be considered a valid measure of C. parvum viability, given that it is biologically implausible for oocysts to be nonviable and yet infectious.

* Corresponding author. Mailing address: Veterinary Medicine Teaching and Research Center, School of Veterinary Medicine, University of California—Davis, Tulare, CA 93274. Phone: (559) 688-1731. Fax: (559) 686-4231. E-mail: ratwill{at}vmtrc.ucdavis.edu.

Applied and Environmental Microbiology, January 2004, p. 642-646, Vol. 70, No. 1
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.1.642-646.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Searcy, K. E., Packman, A. I., Atwill, E. R., Harter, T. (2006). Capture and Retention of Cryptosporidium parvum Oocysts by Pseudomonas aeruginosa Biofilms. Appl. Environ. Microbiol. 72: 6242-6247 [Abstract] [Full Text]  
  • Atwill, E. R., Phillips, R., Pereira, M. D. G. C., Li, X., McCowan, B. (2004). Seasonal Shedding of Multiple Cryptosporidium Genotypes in California Ground Squirrels (Spermophilus beecheyi). Appl. Environ. Microbiol. 70: 6748-6752 [Abstract] [Full Text]  


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