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Applied and Environmental Microbiology, October 2004, p. 6113-6122, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.6113-6122.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Comparison of Compositions and Metabolic Activities of Fecal Microbiotas in Young Adults and in Antibiotic-Treated and Non-Antibiotic-Treated Elderly Subjects

Emma J. Woodmansey,1 Marion E. T. McMurdo,2 George T. Macfarlane,1 and Sandra Macfarlane1*

Microbiology and Gut Biology Group,1 Department of Medicine, Section of Ageing and Health, University of Dundee, Dundee, United Kingdom2

Received 17 November 2003/ Accepted 14 June 2004

The colonic microbiota mediates many cellular and molecular events in the host that are important to health. These processes can be affected in the elderly, because in some individuals, the composition and metabolic activities of the microbiota change with age. Detailed characterizations of the major groups of fecal bacteria in healthy young adults, in healthy elderly people, and in hospitalized elderly patients receiving antibiotics were made in this study, together with measurements of their metabolic activities, by analysis of fecal organic acid and ammonia concentrations. The results showed that total anaerobe numbers remained relatively constant in old people; however, individual bacterial genera changed markedly with age. Reductions in numbers of bacteroides and bifidobacteria in both elderly groups were accompanied by reduced species diversity. Bifidobacterial populations in particular showed marked variations in the dominant species, with Bifidobacterium angulatum and Bifidobacterium adolescentis being frequently isolated from the elderly and Bifidobacterium longum, Bifidobacterium catenulatum, Bifidobacterium boum, and Bifidobacterium infantis being detected only from the healthy young volunteers. Reductions in amylolytic activities of bacterial isolates in healthy elderly subjects and reduced short-chain fatty acid concentrations supported these findings, since bifidobacteria and bacteroides are important saccharolytic groups in the colon. Conversely, higher numbers of proteolytic bacteria were observed with feces samples from the antibiotic-treated elderly group, which were also associated with increased proteolytic species diversity (fusobacteria, clostridia, and propionibacteria). Other differences in the intestinal ecosystem in elderly subjects were observed, with alterations in the dominant clostridial species in combination with greater numbers of facultative anaerobes.

* Corresponding author. Mailing address: Microbiology and Gut Biology Group, Department of Molecular and Cellular Pathology, University of Dundee, Level 6, Ninewells Hospital Medical School, Dundee DD1 9SY, United Kingdom. Phone: 44-1382-632535. Fax: 44-1382-633952. E-mail: s.macfarlane{at}dundee.ac.uk

Applied and Environmental Microbiology, October 2004, p. 6113-6122, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.6113-6122.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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