This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ventura, M.
Right arrow Articles by van Sinderen, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ventura, M.
Right arrow Articles by van Sinderen, D.
Agricola
Right arrow Articles by Ventura, M.
Right arrow Articles by van Sinderen, D.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 2004, p. 6197-6209, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.6197-6209.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Characterization of the groEL and groES Loci in Bifidobacterium breve UCC 2003: Genetic, Transcriptional, and Phylogenetic Analyses

Marco Ventura,1,2* Carlos Canchaya,1 Ralf Zink,3 Gerald F. Fitzgerald,1,2 and Douwe van Sinderen1,2

Department of Microbiology,1 and Alimentary Pharmabiotic Centre,2 Department of Nutrition and Health, Cognis, Germany3

Received 1 April 2004/ Accepted 27 May 2004

The bacterial heat shock response is characterized by the elevated expression of a number of chaperone complexes, including the GroEL and GroES proteins. The groES and groEL genes are highly conserved among eubacteria and are typically arranged as an operon. Genome analysis of Bifidobacterium breve UCC 2003 revealed that the groES and groEL genes are located in different chromosomal regions. The heat inducibility of the groEL and groES genes of B. breve UCC 2003 was verified by slot blot analysis. Northern blot analyses showed that the cspA gene is cotranscribed with the groEL gene, while the groES gene is transcribed as a monocistronic unit. The transcription initiation sites of these two mRNAs were determined by primer extension. Sequence and transcriptional analyses of the region flanking the groEL and groES genes of various bifidobacteria revealed similar groEL-cspA and groES gene units, suggesting a novel genetic organization of these chaperones. Phylogenetic analysis of the available bifidobacterial groES and groEL genes suggested that these genes evolved differently. Discrepancies in the phylogenetic positioning of groES-based trees make this gene an unreliable molecular marker. On the other hand, the bifidobacterial groEL gene sequences can be used as an alternative to current methods for tracing Bifidobacterium species, particularly because they allow a high level of discrimination between closely related species of this genus.

* Corresponding author. Mailing address: Department of Microbiology, National University of Ireland, Western Road, Cork, Ireland. Phone: 353-21-4901366. Fax: 353-21-4903031. E-mail: m.ventura{at}ucc.ie.

Applied and Environmental Microbiology, October 2004, p. 6197-6209, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.6197-6209.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zomer, A., Fernandez, M., Kearney, B., Fitzgerald, G. F., Ventura, M., van Sinderen, D. (2009). An Interactive Regulatory Network Controls Stress Response in Bifidobacterium breve UCC2003. J. Bacteriol. 191: 7039-7049 [Abstract] [Full Text]  
  • Turroni, F., Foroni, E., Pizzetti, P., Giubellini, V., Ribbera, A., Merusi, P., Cagnasso, P., Bizzarri, B., de'Angelis, G. L., Shanahan, F., van Sinderen, D., Ventura, M. (2009). Exploring the Diversity of the Bifidobacterial Population in the Human Intestinal Tract. Appl. Environ. Microbiol. 75: 1534-1545 [Abstract] [Full Text]  
  • Claesson, M. J., van Sinderen, D., O'Toole, P. W. (2008). Lactobacillus phylogenomics - towards a reclassification of the genus. Int. J. Syst. Evol. Microbiol. 58: 2945-2954 [Abstract] [Full Text]  
  • Solano-Aguilar, G., Dawson, H., Restrepo, M., Andrews, K., Vinyard, B., Urban, J. F. Jr. (2008). Detection of Bifidobacterium animalis subsp. lactis (Bb12) in the Intestine after Feeding of Sows and Their Piglets. Appl. Environ. Microbiol. 74: 6338-6347 [Abstract] [Full Text]  
  • Sriramulu, D. D., Liang, M., Hernandez-Romero, D., Raux-Deery, E., Lunsdorf, H., Parsons, J. B., Warren, M. J., Prentice, M. B. (2008). Lactobacillus reuteri DSM 20016 Produces Cobalamin-Dependent Diol Dehydratase in Metabolosomes and Metabolizes 1,2-Propanediol by Disproportionation. J. Bacteriol. 190: 4559-4567 [Abstract] [Full Text]  
  • Okamoto, M., Benno, Y., Leung, K.-P, Maeda, N. (2008). Bifidobacterium tsurumiense sp. nov., from hamster dental plaque. Int. J. Syst. Evol. Microbiol. 58: 144-148 [Abstract] [Full Text]  
  • Sanchez, B., Champomier-Verges, M.-C., Stuer-Lauridsen, B., Ruas-Madiedo, P., Anglade, P., Baraige, F., de los Reyes-Gavilan, C. G., Johansen, E., Zagorec, M., Margolles, A. (2007). Adaptation and Response of Bifidobacterium animalis subsp. lactis to Bile: a Proteomic and Physiological Approach. Appl. Environ. Microbiol. 73: 6757-6767 [Abstract] [Full Text]  
  • Sheehan, V. M., Sleator, R. D., Hill, C., Fitzgerald, G. F. (2007). Improving gastric transit, gastrointestinal persistence and therapeutic efficacy of the probiotic strain Bifidobacterium breve UCC2003. Microbiology 153: 3563-3571 [Abstract] [Full Text]  
  • Ventura, M., Canchaya, C., Tauch, A., Chandra, G., Fitzgerald, G. F., Chater, K. F., van Sinderen, D. (2007). Genomics of Actinobacteria: Tracing the Evolutionary History of an Ancient Phylum. Microbiol. Mol. Biol. Rev. 71: 495-548 [Abstract] [Full Text]  
  • Ventura, M., Canchaya, C., Zhang, Z., Fitzgerald, G. F., van Sinderen, D. (2007). Molecular Characterization of hsp20, Encoding a Small Heat Shock Protein of Bifidobacterium breve UCC2003. Appl. Environ. Microbiol. 73: 4695-4703 [Abstract] [Full Text]  
  • Ventura, M., Canchaya, C., Casale, A. D., Dellaglio, F., Neviani, E., Fitzgerald, G. F., van Sinderen, D. (2006). Analysis of bifidobacterial evolution using a multilocus approach. Int. J. Syst. Evol. Microbiol. 56: 2783-2792 [Abstract] [Full Text]  
  • Li, Y., Raftis, E., Canchaya, C., Fitzgerald, G. F., van Sinderen, D., O'Toole, P. W. (2006). Polyphasic analysis indicates that Lactobacillus salivarius subsp. salivarius and Lactobacillus salivarius subsp. salicinius do not merit separate subspecies status.. Int. J. Syst. Evol. Microbiol. 56: 2397-2403 [Abstract] [Full Text]  
  • Ventura, M., Zhang, Z., Cronin, M., Canchaya, C., Kenny, J. G., Fitzgerald, G. F., van Sinderen, D. (2005). The ClgR Protein Regulates Transcription of the clpP Operon in Bifidobacterium breve UCC 2003. J. Bacteriol. 187: 8411-8426 [Abstract] [Full Text]  
  • Ventura, M., Lee, J.-H., Canchaya, C., Zink, R., Leahy, S., Moreno-Munoz, J. A., O'Connell-Motherway, M., Higgins, D., Fitzgerald, G. F., O'Sullivan, D. J., van Sinderen, D. (2005). Prophage-Like Elements in Bifidobacteria: Insights from Genomics, Transcription, Integration, Distribution, and Phylogenetic Analysis. Appl. Environ. Microbiol. 71: 8692-8705 [Abstract] [Full Text]  
  • Ventura, M., Canchaya, C., Bernini, V., Del Casale, A., Dellaglio, F., Neviani, E., Fitzgerald, G. F., van Sinderen, D. (2005). Genetic Characterization of the Bifidobacterium breve UCC 2003 hrcA Locus. Appl. Environ. Microbiol. 71: 8998-9007 [Abstract] [Full Text]  
  • Ventura, M., Fitzgerald, G. F., van Sinderen, D. (2005). Genetic and Transcriptional Organization of the clpC Locus in Bifidobacterium breve UCC 2003. Appl. Environ. Microbiol. 71: 6282-6291 [Abstract] [Full Text]  
  • Ventura, M., Kenny, J. G., Zhang, Z., Fitzgerald, G. F., van Sinderen, D. (2005). The clpB gene of Bifidobacterium breve UCC 2003: transcriptional analysis and first insights into stress induction. Microbiology 151: 2861-2872 [Abstract] [Full Text]  
  • Sanchez, B., Champomier-Verges, M.-C., Anglade, P., Baraige, F., de los Reyes-Gavilan, C. G., Margolles, A., Zagorec, M. (2005). Proteomic Analysis of Global Changes in Protein Expression during Bile Salt Exposure of Bifidobacterium longum NCIMB 8809. J. Bacteriol. 187: 5799-5808 [Abstract] [Full Text]  
  • Ventura, M., Zink, R., Fitzgerald, G. F., van Sinderen, D. (2005). Gene Structure and Transcriptional Organization of the dnaK Operon of Bifidobacterium breve UCC 2003 and Application of the Operon in Bifidobacterial Tracing. Appl. Environ. Microbiol. 71: 487-500 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»