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Applied and Environmental Microbiology, October 2004, p. 6299-6301, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.6299-6301.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Rapid Quantitative Detection of Listeria monocytogenes in Meat Products by Real-Time PCR

David Rodríguez-Lázaro,1 Anna Jofré,2 Teresa Aymerich,2 Marta Hugas,2,{dagger} and Maria Pla1*

Institute of Food and Agricultural Technology, University of Girona, Girona,1 Institute for Food and Agricultural Research and Technology, Meat Technology Center-IRTA, Monells, Spain2

Received 20 February 2004/ Accepted 31 May 2004

ABSTRACT

We describe a quick and simple method for the quantitative detection of Listeria monocytogenes in meat products. This method is based on filtration, Chelex-100-based DNA purification, and real-time PCR. It can detect as few as 100 CFU/g and quantify as few as 1,000 CFU/g, with excellent accuracy compared to that of the plate count method. Therefore, it is a promising alternative for the detection of L. monocytogenes in meat products.

* Corresponding author. Mailing address: Institute of Food and Agricultural Technology, University of Girona, Campus Montilivi s/n, E-17071 Girona, Spain. Phone: 34-972 419852. Fax: 34-972 418399. E-mail: maria.pla{at}udg.es.

FOOTNOTES

{dagger} Present address: European Food Safety Authority, B-1140 Brussels, Belgium.

Applied and Environmental Microbiology, October 2004, p. 6299-6301, Vol. 70, No. 10
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.10.6299-6301.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lopez-Enriquez, L., Rodriguez-Lazaro, D., Hernandez, M. (2007). Quantitative Detection of Clostridium tyrobutyricum in Milk by Real-Time PCR. Appl. Environ. Microbiol. 73: 3747-3751 [Abstract] [Full Text]  
  • Fukushima, H., Katsube, K., Hata, Y., Kishi, R., Fujiwara, S. (2007). Rapid Separation and Concentration of Food-Borne Pathogens in Food Samples Prior to Quantification by Viable-Cell Counting and Real-Time PCR. Appl. Environ. Microbiol. 73: 92-100 [Abstract] [Full Text]  
  • Rodriguez-Lazaro, D., Pla, M., Scortti, M., Monzo, H. J., Vazquez-Boland, J. A. (2005). A Novel Real-Time PCR for Listeria monocytogenes That Monitors Analytical Performance via an Internal Amplification Control. Appl. Environ. Microbiol. 71: 9008-9012 [Abstract] [Full Text]  


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