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Applied and Environmental Microbiology, November 2004, p. 6401-6406, Vol. 70, No. 11
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.11.6401-6406.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Serologic and Molecular Characterization of Vibrio parahaemolyticus Strains Isolated from Seawater and Fish Products of the Gulf of Mexico

María Eugenia Cabrera-García,1 Carlos Vázquez-Salinas,2* and Elsa Irma Quiñones-Ramírez1

Department of Microbiology of the National School of Biological Sciences, Instituto Politécnico Nacional,1 Department of Biotechnology, Division of Biological and Health Sciences, Universidad Autónoma Metropolitana, Mexico City, Mexico2

Received 12 February 2004/ Accepted 25 June 2004

The thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) are the main virulence factors of Vibrio parahaemolyticus. We isolated V. parahaemolyticus from seawater, fish, and oysters obtained from the Pueblo Viejo Lagoon in Veracruz, determined the serogroups, phenotypically and genotypically characterized TDH and TRH, and investigated the presence of the toxR gene. A total of 46 V. parahaemolyticus strains were isolated, and all of them amplified the 368-bp toxR gene fragment. The trh gene was not identified in any of the strains; 4 of the 46 strains were Kanagawa phenomenon (KP) positive and amplified the 251-bp tdh gene fragment. The most frequent serogroup was serogroup O3. This is the first report of the presence of KP-positive tdh-positive environmental V. parahaemolyticus strains in Mexico.

* Corresponding author. Mailing address: Departamento de Biotecnología, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, Michoacán y Purísima S/N, Col. Vicentina, C.P. 09340, México D. F., Mexico. Phone: (52 55) 5804-4724. Fax: (52 55) 5804-4712. E-mail: cvs{at}xanum.uam.mx.

Applied and Environmental Microbiology, November 2004, p. 6401-6406, Vol. 70, No. 11
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.11.6401-6406.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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