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Applied and Environmental Microbiology, November 2004, p. 6650-6656, Vol. 70, No. 11
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.11.6650-6656.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Dynamics of Multiple lin Gene Expression in Sphingomonas paucimobilis B90A in Response to Different Hexachlorocyclohexane Isomers

Mrutyunjay Suar,¹ Jan Roelof van der Meer,^2,3* Kirsten Lawlor,² Christof Holliger,⁴ and Rup Lal¹

Department of Zoology, University of Delhi, Delhi, India,¹ Process of Environmental Microbiology and Molecular Ecotoxicology, Swiss Federal Institute for Environmental Science and Technology (EAWAG), Duebendorf,² and Laboratory for Environmental Biotechnology, ENAC-ISTE, EPFL,⁴ Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland³

Received 10 March 2004/ Accepted 5 July 2004

Sphingomonas paucimobilis B90A is able to degrade the -, ß-, -, and -isomers of hexachlorocyclohexane (HCH). It contains the genes linA, linB, linC, linD, linE, and linR, which have been implicated in HCH degradation. In this study, dynamic expression of the lin genes was measured in chemostat-grown S. paucimobilis B90A by RNA dot blot hybridization and real-time reverse transcriptase PCR upon exposure to a pulse of different HCH isomers. Irrespective of the addition of HCH, linA, linB, and linC were all expressed constitutively. In contrast, linD and linE were induced with -HCH (2 mg/liter) and -HCH (7 mg/liter). A sharp increase in mRNA levels for linD and linE was observed from 10 to 45 min after the addition of - or -HCH. Induction of linD and linE was not detectable upon the addition of 0.7 mg of -HCH per liter, although the compound was degraded by the cells. The addition of ß-HCH (5 mg/liter) or -HCH (20 mg/liter) did not lead to linE and linD induction, despite the fact that 50% of the compounds were degraded. This suggests that degradation of ß- and -HCH proceeds by a different pathway than that of - and -HCH.

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* Corresponding author. Mailing address: University of Lausanne, Department of Fundamental Microbiology, BÂtiment de Biologie, CH-1015 Lausanne, Switzerland. Phone: 41 21 692 5630. Fax: 41 21 692 5605. E-mail: JanRoelof.VanDerMeer{at}unil.ch.

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Applied and Environmental Microbiology, November 2004, p. 6650-6656, Vol. 70, No. 11
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.11.6650-6656.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

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