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Applied and Environmental Microbiology, November 2004, p. 6920-6926, Vol. 70, No. 11
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.11.6920-6926.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Combined Phospholipid Biomarker-16S rRNA Gene Denaturing Gradient Gel Electrophoresis Analysis of Bacterial Diversity and Physiological Status in an Intertidal Microbial Mat

Laura Villanueva,1* Antoni Navarrete,1 Jordi Urmeneta,1 David C. White,2 and Ricardo Guerrero1

Department of Microbiology, University of Barcelona, Barcelona, Spain,1 Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee2

Received 12 January 2004/ Accepted 26 June 2004

ABSTRACT

A combined lipid biomarker-16S rRNA gene denaturing gradient gel electrophoresis analysis was used to monitor changes in the physiological status, biomass, and microbial composition of a microbial mat. In the morning hours, an increase in the biomass of layers containing a high density of phototrophs and a decrease in the growth rate in the deep layers were observed. The combined approach also revealed differences in major groups of microorganisms, including green nonsulfur, gram-positive, and heterotrophic bacteria.

* Corresponding author. Mailing address: Department of Microbiology, University of Barcelona, Av. Diagonal 645, E-08028 Barcelona, Spain. Phone: 34-934034628. Fax: 34-934034629. E-mail: address: villanueva{at}ub.edu.

Applied and Environmental Microbiology, November 2004, p. 6920-6926, Vol. 70, No. 11
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.11.6920-6926.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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