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Applied and Environmental Microbiology, December 2004, p. 7018-7023, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7018-7023.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Comparison of Two Alternative Dominant Selectable Markers for Wine Yeast Transformation

Eduardo Cebollero and Ramon Gonzalez*

Department of Microbiology, Instituto de Fermentaciones Industriales (CSIC), Madrid, Spain

Received 26 February 2004/ Accepted 6 August 2004

Genetic improvement of industrial yeast strains is restricted by the availability of selectable transformation markers. Antibiotic resistance markers have to be avoided for public health reasons, while auxotrophy markers are generally not useful for wine yeast strain transformation because most industrial Saccharomyces cerevisiae strains are prototrophic. For this work, we performed a comparative study of the usefulness of two alternative dominant selectable markers in both episomic and centromeric plasmids. Even though the selection for sulfite resistance conferred by FZF1-4 resulted in a larger number of transformants for a laboratory strain, the p-fluoro-DL-phenylalanine resistance conferred by ARO4-OFP resulted in a more suitable selection marker for all industrial strains tested. Both episomic and centromeric constructions carrying this marker resulted in transformation frequencies close to or above 103 transformants per µg of DNA for the three wine yeast strains tested.

* Corresponding author. Mailing address: Department of Microbiology, Instituto de Fermentaciones Industriales (CSIC), C. Juan de la Cierva 3, 28006 Madrid, Spain. Phone: 34-915622900(359). Fax: 34-915644853. E-mail: rgonzalez{at}ifi.csic.es.

Applied and Environmental Microbiology, December 2004, p. 7018-7023, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7018-7023.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Gonzalez-Ramos, D., Cebollero, E., Gonzalez, R. (2008). A Recombinant Saccharomyces cerevisiae Strain Overproducing Mannoproteins Stabilizes Wine against Protein Haze. Appl. Environ. Microbiol. 74: 5533-5540 [Abstract] [Full Text]  


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