This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Marceau, A.
Right arrow Articles by Champomier-Vergès, M.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marceau, A.
Right arrow Articles by Champomier-Vergès, M.-C.
Agricola
Right arrow Articles by Marceau, A.
Right arrow Articles by Champomier-Vergès, M.-C.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 2004, p. 7260-7268, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7260-7268.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evidence for Involvement of at Least Six Proteins in Adaptation of Lactobacillus sakei to Cold Temperatures and Addition of NaCl

Anika Marceau, Monique Zagorec, Stéphane Chaillou, Thérèse Méra, and Marie-Christine Champomier-Vergès*

Unité Flore Lactique et Environnement Carné, Institut National de la Recherche Agronomique, Domaine de Vilvert, Jouy en Josas, France

Received 22 April 2004/ Accepted 22 July 2004

Lactobacillus sakei is a lactic acid bacterium widely represented in the natural flora of fresh meat. The aim of this study was to analyze the differences in protein expression during environmental changes encountered during technological processes in which L. sakei is involved in order to gain insight into the ability of this species to grow and survive in such environments. Using two-dimensional electrophoresis, we observed significant variation of a set of 21 proteins in cells grown at 4°C or in the presence of 4% NaCl. Six proteins could be identified by determination of their N-terminal sequences, and the corresponding gene clusters were studied. Two proteins belong to carbon metabolic pathways, and four can be clustered as general stress proteins. A phenotype was observed at low temperature for five of the six mutants constructed for these genes. The survival of four mutants during stationary phase at 4°C was affected, and surprisingly, one mutant showed enhanced survival during stationary phase at low temperatures.

* Corresponding author. Mailing address: Unité Flore Lactique et Environnement Carné, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78350 Jouy en Josas, France. Phone: 33 (0) 1 34 65 22 92. Fax: 33 (0) 1 34 65 21 05. E-mail: verges{at}diamant.jouy.inra.fr.

Applied and Environmental Microbiology, December 2004, p. 7260-7268, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7260-7268.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Chaillou, S., Daty, M., Baraige, F., Dudez, A.-M., Anglade, P., Jones, R., Alpert, C.-A., Champomier-Verges, M.-C., Zagorec, M. (2009). Intraspecies Genomic Diversity and Natural Population Structure of the Meat-Borne Lactic Acid Bacterium Lactobacillus sakei. Appl. Environ. Microbiol. 75: 970-980 [Abstract] [Full Text]  
  • Sanchez, B., Champomier-Verges, M.-C., Anglade, P., Baraige, F., de los Reyes-Gavilan, C. G., Margolles, A., Zagorec, M. (2005). Proteomic Analysis of Global Changes in Protein Expression during Bile Salt Exposure of Bifidobacterium longum NCIMB 8809. J. Bacteriol. 187: 5799-5808 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»