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Applied and Environmental Microbiology, December 2004, p. 7288-7294, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7288-7294.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Seasonal Incidence of Autochthonous Antagonistic Roseobacter spp. and Vibrionaceae Strains in a Turbot Larva (Scophthalmus maximus) Rearing System

Mette Hjelm,1 Ana Riaza,2 Fernanda Formoso,2 Jette Melchiorsen,1 and Lone Gram1*

Department of Seafood Research, Danish Institute for Fisheries Research, Kongens Lyngby, Denmark,1 Stolt Sea Farms SA, Ponta de los Remedios, LIRA, Carnota, La Coruña, Spain2

Received 19 May 2004/ Accepted 27 July 2004

Bacteria inhibitory to fish larval pathogenic bacteria were isolated from two turbot larva rearing farms over a 1-year period. Samples were taken from the rearing site, e.g., tank walls, water, and feed for larvae, and bacteria with antagonistic activity against Vibrio anguillarum were isolated using a replica plating assay. Approximately 19,000 colonies were replica plated from marine agar plates, and 341 strains were isolated from colonies causing clearing zones in a layer of V. anguillarum. When tested in a well diffusion agar assay, 173 strains retained the antibacterial activity against V. anguillarum and Vibrio splendidus. Biochemical tests identified 132 strains as Roseobacter spp. and 31 as Vibrionaceae strains. Partial sequencing of the 16S rRNA gene of three strains confirmed the identification as Roseobacter gallaeciensis. Roseobacter spp. were especially isolated in the spring and early summer months. Subtyping of the 132 Roseobacter spp. strains by randomly amplified polymorphic DNA with two primers revealed that the strains formed a very homogeneous group. Hence, it appears that the same subtype was present at both fish farms and persisted during the 1-year survey. This indicates either a common, regular source of the subtype or the possibility that a particular subtype has established itself in some areas of the fish farm. Thirty-one antagonists were identified as Vibrio spp., and 18 of these were V. anguillarum but not serotype O1 or O2. Roseobacter spp. strains were, in particular, isolated from the larval tank walls, and it may be possible to establish an antagonistic, beneficial microflora in the rearing environment of turbot larvae and thereby limit survival of pathogenic bacteria.

* Corresponding author. Mailing address: Danish Institute for Fisheries Research, Department of Seafood Research, Søltofts Plads, c/o Technical University of Denmark, Bldg. 221, DK-2800 Kgs. Lyngby, Denmark. Phone: 45 45 25 25 86. Fax: 45 45 88 47 74 E-mail: gram{at}dfu.min.dk.

Applied and Environmental Microbiology, December 2004, p. 7288-7294, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7288-7294.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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  • Porsby, C. H., Nielsen, K. F., Gram, L. (2008). Phaeobacter and Ruegeria Species of the Roseobacter Clade Colonize Separate Niches in a Danish Turbot (Scophthalmus maximus)-Rearing Farm and Antagonize Vibrio anguillarum under Different Growth Conditions. Appl. Environ. Microbiol. 74: 7356-7364 [Abstract] [Full Text]  
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