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Applied and Environmental Microbiology, December 2004, p. 7413-7417, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7413-7417.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Cadmium-Responsive Thiols in the Ectomycorrhizal Fungus Paxillus involutus

Mikael Courbot,1 Laurent Diez,2 Roberta Ruotolo,3 Michel Chalot,1* and Pierre Leroy2

Interactions Arbres/Microorganismes, Faculté des Sciences et Techniques,1 Laboratoire Thiols et Fonctions Cellulaires, Faculté de Pharmacie, Université Henri Poincaré-Nancy 1, Nancy, France,2 Dipartimento di Biochimica e Biologia Molecolare, University of Parma, Parma, Italy3

Received 9 April 2004/ Accepted 16 August 2004

Molecular and cellular mechanisms underlying the sustained metal tolerance of ectomycorrhizal fungi are largely unknown. Some of the main mechanisms involved in metal detoxification appear to involve the chelation of metal ions in the cytosol with thiol-containing compounds, such as glutathione, phytochelatins, or metallothioneins. We used an improved high-performance liquid chromatography method for the simultaneous measurement of thiol-containing compounds from cysteine and its derivatives ({gamma}-glutamylcysteine, glutathione) to higher-molecular-mass compounds (phytochelatins). We found that glutathione and {gamma}-glutamylcysteine contents increased when the ectomycorrhizal fungus Paxillus involutus was exposed to cadmium. An additional compound with a 3-kDa molecular mass, most probably related to a metallothionein, increased drastically in mycelia exposed to cadmium. The relative lack of phytochelatins and the presence of a putative metallothionein suggest that ectomycorrhizal fungi may use a different means to tolerate heavy metals, such as Cd, than do their plant hosts.

* Corresponding author. Mailing address: Université Henri Poincaré Nancy I, UMR INRA/UHP 1136, Interactions Arbres/Micro-organismes, B.P. 239, F-54506 Vandoeuvre-les-Nancy cedex, France. Phone: 33 3 83 68 42 38. Fax: 33 3 83 68 42 92. E-mail: Michel.Chalot{at}scbiol.uhp-nancy.fr.

Applied and Environmental Microbiology, December 2004, p. 7413-7417, Vol. 70, No. 12
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.12.7413-7417.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ramesh, G., Podila, G. K., Gay, G., Marmeisse, R., Reddy, M. S. (2009). Different Patterns of Regulation for the Copper and Cadmium Metallothioneins of the Ectomycorrhizal Fungus Hebeloma cylindrosporum. Appl. Environ. Microbiol. 75: 2266-2274 [Abstract] [Full Text]  


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