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Applied and Environmental Microbiology, February 2004, p. 1245-1248, Vol. 70, No. 2
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.2.1245-1248.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

New Approach for Specific Determination of Antibiotics by Use of Luminescent Escherichia coli and Immune Serum

Irina I. Vlasova,1* Tatyana V. Asrieli,2 Elisaveta M. Gavrilova,2 and Vadim S. Danilov1

Department of Microbiology, Faculty of Biology,1 Department of Immunochemistry, Faculty of Chemistry, M. V. Lomonosov Moscow State University, 119899 Moscow, Russia2

Received 3 June 2003/ Accepted 24 October 2003

This paper describes a possible application of luminescent Escherichia coli activated by blood serum for high-sensitivity and high-specificity assays of antibiotics in solutions. Antibiotics inhibited luminescence of a genetically engineered E. coli strain; the system sensitivity to some antibiotics grew notably after the cells had been preactivated by blood serum. The highest level of sensitivity (2.8 ± 0.6 ng/ml) of luminescent cells was obtained for aminoglycoside antibiotics (gentamicin and streptomycin). It is feasible to create the specific biosensor for antibiotics on the basis of bioluminescent E. coli strains by applying sera containing antibodies against the antibiotic under assay. The presence of antibodies specific for gentamicin in serum affects inhibition of luminescent cells by gentamicin but not inhibition by other antibiotics.

* Corresponding author. Mailing address: Faculty of Biology, Moscow State University, 119899 Moscow, Russia. Phone and fax: 7-095-246-44-90. E-mail: Irina-Vlasova{at}newmail.ru.

Applied and Environmental Microbiology, February 2004, p. 1245-1248, Vol. 70, No. 2
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.2.1245-1248.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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