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Applied and Environmental Microbiology, February 2004, p. 664-669, Vol. 70, No. 2
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.2.664-669.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Diversity of Phage Types among Archived Cultures of the Demerec Collection of Salmonella enterica serovar Typhimurium Strains{dagger}

Wolfgang Rabsch,1 R. Allen Helm,2 and Abraham Eisenstark2,3*

National Reference Centre for Salmonella and Other Enterics, Robert Koch Institute, Wernigerode Branch, D-38855 Wernigerode, Germany,1 Cancer Research Center, Columbia, Missouri 65201,2 University of Missouri, Columbia, Missouri 652113

Received 23 October 2003/ Accepted 13 November 2003

The existence of several thousand Salmonella enterica serovar Typhimurium LT2 and LT7 cultures originally collected by M. Demerec and sealed in agar stab vials for 33 to 46 years is a resource for evolutionary and mutational studies. Cultures from 74 of these vials, descendants of cells sealed and stored in nutrient agar stabs several decades ago, were phage typed by the Callow and Felix, Lilleengen, and Anderson systems. Among 53 LT2 archived strains, 16 had the same phage type as the nonarchival sequenced LT2 strain. The other 37 archived cultures differed in phage typing pattern from the sequenced strain. These 37 strains were divided into 10 different phage types. Among the 19 LT7 strains, only one was similar to the parent by phage typing, while 18 were different. These 18 strains fell into eight different phage types. The typing systems were developed to track epidemics from source to consumer, as well as geographic spread. The value of phage typing is dependent upon the stability of the phage type of any given strain throughout the course of the investigation. Thus, the variation over time observed in these archived cultures is particularly surprising. Possible mechanisms for such striking diversity may include loss of prophages, prophage mosaics as a result of recombination events, changes in phage receptor sites on the bacterial cell surface, or mutations in restriction-modification systems.

* Corresponding author. Mailing address: Cancer Research Center, 3501 Berrywood Dr., Columbia, MO 65201. Phone: (573) 875-2255. Fax: (573) 443-1202. E-mail: eisenstarka{at}missouri.edu.

{dagger} This paper is dedicated to the memory of Philip and Zlata Hartman, pioneers in Salmonella genetics.

Applied and Environmental Microbiology, February 2004, p. 664-669, Vol. 70, No. 2
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.2.664-669.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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