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Applied and Environmental Microbiology, February 2004, p. 675-678, Vol. 70, No. 2
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.2.675-678.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Observing Growth and Division of Large Numbers of Individual Bacteria by Image Analysis

A. Elfwing,1 Y. LeMarc,2 J. Baranyi,2 and A. Ballagi1*

Center for Surface Biotechnology, University of Uppsala, S-751 23 Uppsala, Sweden,1 Institute of Food Research, Colney, Norwich NR4 7UA, United Kingdom2

Received 8 July 2003/ Accepted 11 November 2003

We describe a method that enabled us to observe large numbers of individual bacterial cells during a long period of cell growth and proliferation. We designed a flow chamber in which the cells attached to a transparent solid surface. The flow chamber was mounted on a microscope equipped with a digital camera. The shear force of the flow removed the daughter cells, making it possible to monitor the consecutive divisions of a single cell. In this way, kinetic parameters and their distributions, as well as some physiological characteristics of the bacteria, could be analyzed based on more than 1,000 single-cell observations. The method which we developed enabled us to study the history effect on the distribution of the lag times of single cells.

* Corresponding author. Mailing address: Center for Surface Biotechnology, University of Uppsala, Husargatan 3, P.O. Box 577, S-751 23 Uppsala, Sweden. Phone: 018 471 00 00. Fax: 018 55 50 16. E-mail: andras.ballagi{at}ytbioteknik.uu.se.

Applied and Environmental Microbiology, February 2004, p. 675-678, Vol. 70, No. 2
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.2.675-678.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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