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Applied and Environmental Microbiology, February 2004, p. 781-789, Vol. 70, No. 2
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.2.781-789.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Phylogenetic Composition of Arctic Ocean Archaeal Assemblages and Comparison with Antarctic Assemblages

Nasreen Bano, Shomari Ruffin, Briana Ransom, and James T. Hollibaugh*

Department of Marine Sciences, University of Georgia, Athens, Georgia 30602-3636

Received 2 July 2003/ Accepted 17 November 2003

Archaea assemblages from the Arctic Ocean and Antarctic waters were compared by PCR-denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA genes amplified using the Archaea-specific primers 344f and 517r. Inspection of the DGGE fingerprints of 33 samples from the Arctic Ocean (from SCICEX submarine cruises in 1995, 1996, and 1997) and 7 Antarctic samples from Gerlache Strait and Dallman Bay revealed that the richness of Archaea assemblages was greater in samples from deep water than in those from the upper water column in both polar oceans. DGGE banding patterns suggested that most of the Archaea ribotypes were common to both the Arctic Ocean and the Antarctic Ocean. However, some of the Euryarchaeota ribotypes were unique to each system. Cluster analysis of DGGE fingerprints revealed no seasonal variation but supported depth-related differences in the composition of the Arctic Ocean Archaea assemblage. The phylogenetic composition of the Archaea assemblage was determined by cloning and then sequencing amplicons obtained from the Archaea-specific primers 21f and 958r. Sequences of 198 clones from nine samples covering three seasons and all depths grouped with marine group I Crenarchaeota (111 clones), marine group II Euryarchaeota (86 clones), and group IV Euryarchaeota (1 clone). A sequence obtained only from a DGGE band was similar to those of the marine group III Euryarchaeota.

* Corresponding author. Mailing address: Department of Marine Sciences, University of Georgia, Athens, GA 30602-3636. Phone: (706) 542-5868. Fax: (706) 542-5888. E-mail: aquadoc{at}uga.edu.

Applied and Environmental Microbiology, February 2004, p. 781-789, Vol. 70, No. 2
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.2.781-789.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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