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Applied and Environmental Microbiology, February 2004, p. 845-849, Vol. 70, No. 2
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.2.845-849.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

New Perspective on Uncultured Bacterial Phylogenetic Division OP11

J. Kirk Harris,1,2 Scott T. Kelley,1,{dagger} and Norman R. Pace1*

Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347,1 Graduate Group in Microbiology, University of California, Berkeley, California 947202

Received 25 July 2003/ Accepted 23 October 2003

Organisms belonging to the OP11 candidate phylogenetic division of Bacteria have been detected only in rRNA-based sequence surveys of environmental samples. Preliminary studies indicated that such organisms represented by the sequences are abundant and widespread in nature and highly diverse phylogenetically. In order to document more thoroughly the phylogenetic breadth and environmental distribution of this diverse group of organisms, we conducted further molecular analyses on environmental DNAs. Using PCR techniques and primers directed toward each of the five described subdivisions of OP11, we surveyed 17 environmental DNAs and analyzed rRNA gene sequences in 27 clonal libraries from 14 environments. Ninety-nine new and unique sequences were determined completely, and approximately 200 additional clones were subjected to partial sequencing. Extensive phylogenetic comparisons of the new sequences to those representing other bacterial divisions further resolved the phylogeny of the bacterial candidate division OP11 and identified two new candidate bacterial divisions, OP11-derived 1 (OD1) and Sulphur River 1 (SR1). The widespread environmental distribution of representatives of the bacterial divisions OD1, OP11, and SR1 suggests potentially conspicuous biogeochemical roles for these organisms in their respective environments. The information on environmental distribution offers clues for attempts to culture landmark representatives of these novel bacterial divisions, and the sequences are specific molecular signatures that provide for their identification in other contexts.

* Corresponding author. Mailing address: Department of Molecular, Cellular and Developmental Biology, University of Colorado, Campus Box 347, Boulder, CO 80309-0347. Phone: (303) 735-1864. Fax: (303) 492-7744. E-mail: nrpace{at}colorado.edu.

{dagger} Present address: San Diego State University, San Diego, CA 92182.

Applied and Environmental Microbiology, February 2004, p. 845-849, Vol. 70, No. 2
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.2.845-849.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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