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Applied and Environmental Microbiology, March 2004, p. 1717-1722, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1717-1722.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Virucidal Efficacy of Glutaraldehyde against Enteroviruses Is Related to the Location of Lysine Residues in Exposed Structures of the VP1 Capsid Protein

Martine Chambon,1* Christine Archimbaud,1 Jean-Luc Bailly,1 Jeanne-Marie Gourgand,2 Françoise Charbonné,1 and Hélène Peigue-Lafeuille1

Laboratoire de Virologie, Faculté de Médecine, 63001 Clermont-Ferrand Cédex,1 Laboratoire d’Informatique, de Modélisation et d’Optimisation des Systémes, CNRS-UMR 6158, Université Blaise Pascal, 63173 Aubière Cédex, France2

Received 27 May 2003/ Accepted 29 October 2003

Glutaraldehyde (GTA) is a potent virucidal disinfectant whose exact mode of action against enteroviruses is not understood. Earlier reports showed that GTA reacts preferentially with the VP1 capsid protein of echovirus 25 and poliovirus 1 and that GTA has affinity for exposed lysine residues on proteins. To investigate further the inactivation of enteroviruses by GTA, seven strains were selected on the basis of differences in their overall number and the positions of lysine residues in the amino acid sequences of the VP1 polypeptide. Inactivation kinetics experiments were performed with 0.10% GTA. The viruses grouped into three clusters and exhibited significantly different levels of sensitivity to GTA. The results were analyzed in the light of current knowledge of the three-dimensional structure of enteroviruses and the viral life cycle. The differences observed in sensitivity to GTA were related to the number of lysine residues and their locations in the VP1 protein. The overall findings suggest that the BC and DE loops, which cluster at the fivefold axis of symmetry and are the most exposed on the outer surface of the virions, are primary reactive sites for GTA.

* Corresponding author. Mailing address: Laboratoire de Virologie, Faculté de Médecine, 28 place Henri Dunant, 63000 Clermont-Ferrand Cedex 1, France. Phone: 33 4 73 17 81 40. Fax: 33 4 73 44 90 29. E-mail: martine.chambon{at}u-clermont1.fr.

Applied and Environmental Microbiology, March 2004, p. 1717-1722, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1717-1722.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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