This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Walker, M.
Right arrow Articles by Redelman, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Walker, M.
Right arrow Articles by Redelman, D.
Agricola
Right arrow Articles by Walker, M.
Right arrow Articles by Redelman, D.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2004, p. 1827-1829, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1827-1829.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Detection of Cryptosporidium parvum in Soil Extracts{dagger}

Mark Walker1* and Douglas Redelman2

Department of Natural Resources and Environmental Sciences,1 Sierra Cytometry and University of Nevada Cytometry Center, University of Nevada, Reno, Nevada 895572

Received 21 August 2003/ Accepted 17 November 2003

Epifluorescent microscopy and flow cytometry were used in different combinations with fluorescein isothiocyanate-labeled immunoglobulins M and G3 to estimate the numbers of Cryptosporidium parvum oocysts in soil extracts containing 10 to 10,017 oocysts/ml. No combination had a systematic effect on accuracy or precision. Background debris may have produced overestimates at low oocyst concentrations when flow cytometry was used.

* Corresponding author. Mailing address: Department of Natural Resources and Environmental Sciences, MS 370, FA132, University of Nevada, Reno, NV 89557-0013. Phone: (775) 784-1938. Fax: (775) 784-4789. E-mail: mwalker{at}equinox.unr.edu.

{dagger} Publication 52031318 of the Nevada Agricultural Experiment Station.

Applied and Environmental Microbiology, March 2004, p. 1827-1829, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1827-1829.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»