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Applied and Environmental Microbiology, March 2004, p. 1855-1857, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1855-1857.2004

Evaluation of a Real-Time PCR Kit for Detecting Escherichia coli O157 in Bovine Fecal Samples

James L. Bono,1* James E. Keen, Laura C. Miller, James M. Fox, Carol G. Chitko-McKown, Michael P. Heaton, and William W. Laegreid

U.S. Meat Animal Research Center, Agricultural Research Service, U.S. Department of Agriculture, Clay Center, Nebraska 68933-0166

Received 30 July 2003/ Accepted 8 December 2003

A commercially available real-time, rapid PCR test was evaluated for its ability to detect Escherichia coli O157. Both the sensitivity and specificity of the assay were 99% for isolates in pure culture. The assay detected 1 CFU of E. coli O157:H7 g-1 in artificially inoculated bovine feces following enrichment.

* Corresponding author. Mailing address: U.S. Department of Agriculture—ARS, U.S. Meat Animal Research Center, P.O. Box 166, State Spur 18D, Clay Center, NE 68933-0166. Phone: (402) 762-4363. Fax: (402) 762-4375. E-mail: bono{at}email.marc.usda.gov.

Applied and Environmental Microbiology, March 2004, p. 1855-1857, Vol. 70, No. 3
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.3.1855-1857.2004






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