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Applied and Environmental Microbiology, April 2004, p. 1990-1998, Vol. 70, No. 4
0099-2240/04/$08.00+0 DOI: 10.1128/AEM.70.4.1990-1998.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
Cross Talk between 2,4-Diacetylphloroglucinol-Producing Biocontrol Pseudomonads on Wheat Roots
Monika Maurhofer,1 Eric Baehler,2 Regina Notz,1 Vicente Martinez,1 and Christoph Keel2*Institut für Pflanzenwissenschaften/Phytopathologie, Eidgenössische Technische Hochschule, CH-8092 Zürich,1 Département de Microbiologie Fondamentale, Université de Lausanne, CH-1015 Lausanne, Switzerland2
Received 25 September 2003/ Accepted 14 January 2004
The performance of Pseudomonas biocontrol agents may be improved by applying mixtures of strains which are complementary in their capacity to suppress plant diseases. Here, we have chosen the combination of Pseudomonas fluorescens CHA0 with another well-characterized biocontrol agent, P. fluorescens Q2-87, as a model to study how these strains affect each other's expression of a biocontrol trait. In both strains, production of the antimicrobial compound 2,4-diacetylphloroglucinol (DAPG) is a crucial factor contributing to the suppression of root diseases. DAPG acts as a signaling compound inducing the expression of its own biosynthetic genes. Experimental setups were developed to investigate whether, when combining strains CHA0 and Q2-87, DAPG excreted by one strain may influence expression of DAPG-biosynthetic genes in the other strain in vitro and on the roots of wheat. DAPG production was monitored by observing the expression of lacZ fused to the biosynthetic gene phlA of the respective strain. Dual-culture assays in which the two strains were grown in liquid medium physically separated by a membrane revealed that Q2-87 but not its DAPG-negative mutant Q2-87::Tn5-1 strongly induced phlA expression in a 
phlA mutant of strain CHA0. In the same way, phlA expression in a Q2-87 background was induced by DAPG produced by CHA0. When coinoculated onto the roots of wheat seedlings grown under gnotobiotic conditions, strains Q2-87 and CHA0, but not their respective DAPG-negative mutants, were able to enhance phlA expression in each other. In summary, we have established that two nonrelated pseudomonads may stimulate each other in the expression of an antimicrobial compound important for biocontrol. This interpopulation communication occurs in the rhizosphere, i.e., at the site of pathogen inhibition, and is mediated by the antimicrobial compound itself acting as a signal exchanged between the two pseudomonads.
* Corresponding author. Mailing address: Département de Microbiologie Fondamentale, BÂtiment de Biologie, Université de Lausanne, CH-1015 Lausanne, Switzerland. Phone: (41-21) 692-5636. Fax: (41-21) 692-5635. E-mail: christoph.keel{at}imf.unil.ch.
Applied and Environmental Microbiology, April 2004, p. 1990-1998, Vol. 70, No. 4
0099-2240/04/$08.00+0 DOI: 10.1128/AEM.70.4.1990-1998.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.
This article has been cited by other articles:
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de Werra, P., Baehler, E., Huser, A., Keel, C., Maurhofer, M.
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