This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Béra-Maillet, C.
Right arrow Articles by Forano, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Béra-Maillet, C.
Right arrow Articles by Forano, E.
Agricola
Right arrow Articles by Béra-Maillet, C.
Right arrow Articles by Forano, E.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, April 2004, p. 2172-2179, Vol. 70, No. 4
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.4.2172-2179.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Fiber-Degrading Systems of Different Strains of the Genus Fibrobacter

Christel Béra-Maillet,* Yves Ribot, and Evelyne Forano*

Unité de Microbiologie, INRA CR de Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle, France

Received 26 September 2003/ Accepted 27 December 2003

The S85 type strain of Fibrobacter succinogenes, a major ruminal fibrolytic species, was isolated 49 years ago from a bovine rumen and has been used since then as a model for extensive studies. To assess the validity of this model, we compared the cellulase- and xylanase-degrading activities of several other F. succinogenes strains originating from different ruminants, including recently isolated strains, and looked for the presence of 10 glycoside hydrolase genes previously identified in S85. The NR9 F. intestinalis type strain, representative of the second species of the genus, was also included in this study. DNA-DNA hybridization and 16S rRNA gene sequencing first classified the strains and provided the phylogenetic positions of isolates of both species. Cellulase and xylanase activity analyses revealed similar activity profiles for all F. succinogenes strains. However, the FE strain, phylogenetically close to S85, presented a poor xylanolytic system and weak specific activities. Furthermore, the HM2 strain, genetically distant from the other F. succinogenes isolates, displayed a larger cellulolytic profile on zymograms and higher cellulolytic specific activity. F. intestinalis NR9 presented a higher cellulolytic specific activity and a stronger extracellular xylanolytic activity. Almost all glycoside hydrolase genes studied were found in the F. succinogenes isolates by PCR, except in the HM2 strain, and few of them were detected in F. intestinalis NR9. As expected, the fibrolytic genes of strains of the genus Fibrobacter as well as the cellulase and xylanase activities are better conserved in closely related phylogenetic isolates.

* Corresponding author. Mailing address: Unité de Microbiologie, INRA CR de Clermont-Ferrand-Theix, 63122 Saint-Genès-Champanelle, France. Phone: 33-473-62-42-41. Fax: 33-473-62-45-81. E-mail for C. Béra-Maillet: cmaillet{at}clermont.inra.fr; e-mail for E. Forano: forano{at}clermont.inra.fr.

Applied and Environmental Microbiology, April 2004, p. 2172-2179, Vol. 70, No. 4
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.4.2172-2179.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Qi, M., Nelson, K. E., Daugherty, S. C., Nelson, W. C., Hance, I. R., Morrison, M., Forsberg, C. W. (2008). Genomic Differences between Fibrobacter succinogenes S85 and Fibrobacter intestinalis DR7, Identified by Suppression Subtractive Hybridization. Appl. Environ. Microbiol. 74: 987-993 [Abstract] [Full Text]  
  • Jun, H.-S., Qi, M., Gong, J., Egbosimba, E. E., Forsberg, C. W. (2007). Outer Membrane Proteins of Fibrobacter succinogenes with Potential Roles in Adhesion to Cellulose and in Cellulose Digestion. J. Bacteriol. 189: 6806-6815 [Abstract] [Full Text]  
  • Qi, M., Jun, H.-S., Forsberg, C. W. (2007). Characterization and Synergistic Interactions of Fibrobacter succinogenes Glycoside Hydrolases. Appl. Environ. Microbiol. 73: 6098-6105 [Abstract] [Full Text]  
  • Shinkai, T., Kobayashi, Y. (2007). Localization of Ruminal Cellulolytic Bacteria on Plant Fibrous Materials as Determined by Fluorescence In Situ Hybridization and Real-Time PCR. Appl. Environ. Microbiol. 73: 1646-1652 [Abstract] [Full Text]  
  • Qi, M., Nelson, K. E., Daugherty, S. C., Nelson, W. C., Hance, I. R., Morrison, M., Forsberg, C. W. (2005). Novel Molecular Features of the Fibrolytic Intestinal Bacterium Fibrobacter intestinalis Not Shared with Fibrobacter succinogenes as Determined by Suppressive Subtractive Hybridization. J. Bacteriol. 187: 3739-3751 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»