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Applied and Environmental Microbiology, May 2004, p. 2791-2800, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.2791-2800.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Spatial Distribution and Stability of the Eight Microbial Species of the Altered Schaedler Flora in the Mouse Gastrointestinal Tract

Ramahi B. Sarma-Rupavtarm,1 Zhongming Ge,2 David B. Schauer,2,3 James G. Fox,2,3 and Martin F. Polz1*

Department of Civil and Environmental Engineering,1 Division of Biological Engineering,3 Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts2

Received 2 September 2003/ Accepted 11 February 2004

The overall complexity of the microbial communities in the gastrointestinal (GI) tracts of mammals has hindered observations of dynamics and interactions of individual bacterial populations. However, such information is crucial for understanding the diverse disease-causing and protective roles that gut microbiota play in their hosts. Here, we determine the spatial distribution, interanimal variation, and persistence of bacteria in the most complex defined-flora (gnotobiotic) model system to date, viz., mice colonized with the eight strains of the altered Schaedler flora (ASF). Quantitative PCR protocols based on the 16S rRNA sequence of each ASF strain were developed and optimized to specifically detect as few as 10 copies of each target. Total numbers of the ASF strains were determined in the different regions of the GI tracts of three C.B-17 SCID mice. Individual strain abundance was dependent on oxygen sensitivity, with microaerotolerant Lactobacillus murinus ASF361 present at 105 to 107 cells/g of tissue in the upper GI tract and obligate anaerobic ASF strains being predominant in the cecal and colonic flora at 108 to 1010 cells/g of tissue. The variation between the three mice was small for most ASF strains, except for Clostridium sp. strain ASF502 and Bacteroides sp. strain ASF519 in the cecum. A comparison of the relative distribution of the ASF strains in feces and the colon indicated large differences, suggesting that fecal bacterial levels may provide a poor approximation of colonic bacterial levels. All ASF strains were detected by PCR in the feces of C57BL/6 restricted flora mice, which had been maintained in an isolator without sterile food, water, or bedding for several generations, providing evidence for the stability of these strains in the face of potential competition by bacteria introduced into the gut.

* Corresponding author. Mailing address: Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Building 48-417, 77 Massachusetts Ave., Cambridge, MA 02139. Phone: (617) 253-7128. Fax: (617) 258-8850. E-mail: mpolz{at}mit.edu.

Applied and Environmental Microbiology, May 2004, p. 2791-2800, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.2791-2800.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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