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Applied and Environmental Microbiology, May 2004, p. 3138-3142, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.3138-3142.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

pmoA-Based Analysis of Methanotrophs in a Littoral Lake Sediment Reveals a Diverse and Stable Community in a Dynamic Environment

Michael Pester,1 Michael W. Friedrich,2 Bernhard Schink,1 and Andreas Brune1*

Microbial Ecology, Department of Biology, University of Konstanz, 78457 Konstanz,1 Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany2

Received 5 December 2003/ Accepted 18 February 2004

Diversity and community structure of aerobic methane-oxidizing bacteria in the littoral sediment of Lake Constance was investigated by cloning analysis and terminal restriction fragment length polymorphism (T-RFLP) fingerprinting of the pmoA gene. Phylogenetic analysis revealed a high diversity of type I and type II methanotrophs in the oxygenated uppermost centimeter of the sediment. T-RFLP profiles indicated a high similarity between the active methanotrophic community in the oxic layer and the inactive community in an anoxic sediment layer at a 10-cm depth. There were also no major changes in community structure between littoral sediment cores sampled in summer and winter. By contrast, the fingerprint patterns showed substantial differences between the methanotrophic communities of littoral and profundal sediments.

* Corresponding author. Present address: Max Planck Institute for Terrestrial Microbiology, Karl-von-Frisch-Straße, 35043 Marburg, Germany. Phone: 49-6421-178101. Fax: 49-6421-178999. E-mail: brune{at}staff.uni-marburg.de.

Applied and Environmental Microbiology, May 2004, p. 3138-3142, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.3138-3142.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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