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Applied and Environmental Microbiology, May 2004, p. 3143-3145, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.3143-3145.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Plastic Encapsulation of Stabilized Escherichia coli and Pseudomonas putida

M. Manzanera ,1,{dagger},{ddagger} S. Vilchez,2,{dagger} and A. Tunnacliffe1*

Institute of Biotechnology, University of Cambridge, Cambridge CB2 1QT,1 Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom2

Received 31 October 2003/ Accepted 15 January 2004

Escherichia coli and Pseudomonas putida dried in hydroxyectoine or trehalose are shown to be highly resistant to the organic solvents chloroform and acetone, and consequently, they can be encapsulated in a viable form in solid plastic materials. Bacteria are recovered by rehydration after physical disruption of the plastic. P. putida incorporated into a plastic coating of maize seeds was shown to colonize roots efficiently after germination.

* Corresponding author. Mailing address: Institute of Biotechnology, University of Cambridge, Tennis Court Rd., Cambridge CB2 1QT, United Kingdom. Phone: 44-1223-766549. Fax: 44-1223-334162. E-mail: a.tunnacliffe{at}biotech.cam.ac.uk.

{dagger} M.M. and S.V. contributed equally to this work.

{ddagger} Present address: Department of Chemistry, University of Cambridge, Cambridge CB2 1EW, United Kingdom.

Applied and Environmental Microbiology, May 2004, p. 3143-3145, Vol. 70, No. 5
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.5.3143-3145.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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