This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Van Immerseel, F.
Right arrow Articles by Ducatelle, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Van Immerseel, F.
Right arrow Articles by Ducatelle, R.
Agricola
Right arrow Articles by Van Immerseel, F.
Right arrow Articles by Ducatelle, R.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, June 2004, p. 3582-3587, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3582-3587.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Medium-Chain Fatty Acids Decrease Colonization and Invasion through hilA Suppression Shortly after Infection of Chickens with Salmonella enterica Serovar Enteritidis

F. Van Immerseel,1* J. De Buck,1 F. Boyen,1 L. Bohez,1 F. Pasmans,1 J. Volf,2 M. Sevcik,2 I. Rychlik,2 F. Haesebrouck,1 and R. Ducatelle1

Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, B-9820 Merelbeke, Belgium,1 Veterinary Research Institute, 621 32 Brno, Czech Republic2

Received 3 November 2003/ Accepted 1 March 2004

The most common source of Salmonella infections in humans is food of poultry origin. Salmonella enterica serovar Enteritidis has a particular affinity for the contamination of the egg supply. In this study, the medium-chain fatty acids (MCFA), caproic, caprylic, and capric acid, were evaluated for the control of Salmonella serovar Enteritidis in chickens. All MCFA were growth inhibiting at low concentrations in vitro, with caproic acid being the most potent. Contact of Salmonella serovar Enteritidis with low concentrations of MCFA decreased invasion in the intestinal epithelial cell line T84. By using transcriptional fusions between the promoter of the regulatory gene of the Salmonella pathogenicity island I, hilA, and luxCDABE genes, it was shown that all MCFA decreased the expression of hilA, a key regulator related to the invasive capacity of Salmonella. The addition of caproic acid (3 g/kg of feed) to the feed of chicks led to a significant decrease in the level of colonization of ceca and internal organs by Salmonella serovar Enteritidis at 3 days after infection of 5-day-old chicks. These results suggest that MCFA have a synergistic ability to suppress the expression of the genes required for invasion and to reduce the numbers of bacteria in vivo. Thus, MCFA are potentially useful products for reducing the level of colonization of chicks and could ultimately aid in the reduction of the number of contaminated eggs in the food supply.

* Corresponding author. Mailing address: Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. Phone: 32 9 264 74 48. Fax: 32 9 264 74 94. E-mail: filip.vanimmerseel{at}UGent.be.

Applied and Environmental Microbiology, June 2004, p. 3582-3587, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3582-3587.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • De Vylder, J., Van Hoorebeke, S., Ducatelle, R., Pasmans, F., Haesebrouck, F., Dewulf, J., Van Immerseel, F. (2009). Effect of the housing system on shedding and colonization of gut and internal organs of laying hens with Salmonella Enteritidis. Poult. Sci. 88: 2491-2495 [Abstract] [Full Text]  
  • de los Santos, F. S., Donoghue, A. M., Venkitanarayanan, K., Metcalf, J. H., Reyes-Herrera, I., Dirain, M. L., Aguiar, V. F., Blore, P. J., Donoghue, D. J. (2009). The natural feed additive caprylic acid decreases Campylobacter jejuni colonization in market-aged broiler chickens. Poult. Sci. 88: 61-64 [Abstract] [Full Text]  
  • de los Santos, F. S., Donoghue, A. M., Venkitanarayanan, K., Dirain, M. L., Reyes-Herrera, I., Blore, P. J., Donoghue, D. J. (2008). Caprylic Acid Supplemented in Feed Reduces Enteric Campylobacter jejuni Colonization in Ten-Day-Old Broiler Chickens,. Poult. Sci. 87: 800-804 [Abstract] [Full Text]  
  • Gantois, I., Ducatelle, R., Pasmans, F., Haesebrouck, F., Hautefort, I., Thompson, A., Hinton, J. C., Van Immerseel, F. (2006). Butyrate Specifically Down-Regulates Salmonella Pathogenicity Island 1 Gene Expression. Appl. Environ. Microbiol. 72: 946-949 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»