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Applied and Environmental Microbiology, June 2004, p. 3733-3735, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3733-3735.2004

Methods for Enhanced Culture Recovery of Francisella tularensis

Jeannine M. Petersen,* Martin E. Schriefer, Kenneth L. Gage, John A. Montenieri, Leon G. Carter, Miles Stanley, and May C. Chu

Bacterial Zoonoses Branch, Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522

Received 4 November 2003/ Accepted 1 March 2004

Francisella tularensis is found in a wide variety of hosts and extrahost environments, making culture recovery a diagnostic challenge. Here we demonstrate improved recovery times and good sensitivity (90%) when cultures were inoculated on the site of an investigation using fresh tissues. For contaminated specimens, antibiotic supplementation of enriched cysteine heart agar blood culture medium improved recovery of F. tularensis by 81.1%. For transport of tissues, immediate freezing yielded culture recovery rates as high as 94%.

* Corresponding author. Mailing address: Diagnostic and Reference Section, Bacterial Zoonoses Branch, Centers for Disease Control and Prevention, P.O. Box 2087, Rampart Rd., Foothills Campus, Fort Collins, CO 80522. Phone: (970) 266-3524. Fax: (970) 221-6476. E-mail: nzp0{at}cdc.gov.

Applied and Environmental Microbiology, June 2004, p. 3733-3735, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3733-3735.2004




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