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Applied and Environmental Microbiology, June 2004, p. 3751-3753, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3751-3753.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Optimization of Procedures for Isolation of Mycobacteria from Soil and Water Samples Obtained in Northern India

Deepti Parashar,1 D. S. Chauhan,1 V. D. Sharma,1 Aradhana Chauhan,1 S. V. S. Chauhan,2 and V. M. Katoch1*

Central JALMA Institute for Leprosy and Other Mycobacterial Diseases (Indian Council of Medical Research),1 School of Life Sciences, Dr. B. R. Ambedkar University, Agra, India2

Received 14 November 2003/ Accepted 25 February 2004

For isolation of environmental mycobacteria, a decontamination procedure has been standardized by which treatment with 3% sodium dodecyl sulfate plus 4% NaOH (15 and 30 min for rapid and slow growers, respectively) is followed by incubation with 2% cetrimide (5 and 15 min for fast- and slow-growing mycobacteria, respectively); this procedure was found to completely eliminate contamination with other organisms and resulted in the isolation of only mycobacteria.

* Corresponding author. Mailing address: Central JALMA Institute for Leprosy and Other Mycobacterial Diseases (ICMR), Tajganj, Agra 28201, India. Phone: 91-562-2331756. Fax: 91-562-2331755. E-mail: jalma{at}sancharnet.in.

Applied and Environmental Microbiology, June 2004, p. 3751-3753, Vol. 70, No. 6
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.6.3751-3753.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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