This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Connerton, P. L.
Right arrow Articles by Connerton, I. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Connerton, P. L.
Right arrow Articles by Connerton, I. F.
Agricola
Right arrow Articles by Connerton, P. L.
Right arrow Articles by Connerton, I. F.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, July 2004, p. 3877-3883, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.3877-3883.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Longitudinal Study of Campylobacter jejuni Bacteriophages and Their Hosts from Broiler Chickens

P. L. Connerton,1 C. M. Loc Carrillo,1 C. Swift,2 E. Dillon,1 A. Scott,1 C. E. D. Rees,1 C. E. R. Dodd,1 J. Frost,2 and I. F. Connerton1*

Division of Food Sciences, School of Biosciences, Sutton Bonington Campus, University of Nottingham, Loughborough LE12 5RD,1 Laboratory of Enteric Pathogens, Health Protection Agency, London NW9 5HT, United Kingdom2

Received 24 November 2003/ Accepted 29 March 2004

A longitudinal study of bacteriophages and their hosts was carried out at a broiler house that had been identified as having a population of Campylobacter-specific bacteriophages. Cloacal and excreta samples were collected from three successive broiler flocks reared in the same barn. Campylobacter jejuni was isolated from each flock, whereas bacteriophages could be isolated from flocks 1 and 2 but were not isolated from flock 3. The bacteriophages isolated from flocks 1 and 2 were closely related to each other in terms of host range, morphology, genome size, and genetic content. All Campylobacter isolates from flock 1 were genotypically indistinguishable by pulsed-field gel electrophoresis (PFGE). PFGE and multilocus sequence typing indicated that this C. jejuni type was maintained from flock 1 to flock 2 but was largely superseded by three genetically distinct C. jejuni types insensitive to the resident bacteriophages. All isolates from the third batch of birds were insensitive to bacteriophages and genotypically distinct. These results are significant because this is the first study of an environmental population of C. jejuni bacteriophages and their influence on the Campylobacter populations of broiler house chickens. The role of developing bacteriophage resistance was investigated as this is a possible obstacle to the use of bacteriophage therapy to reduce the numbers of campylobacters in chickens. In this broiler house succession was largely due to incursion of new genotypes rather than to de novo development of resistance.

* Corresponding author. Mailing address: Division of Food Sciences, School of Biosciences, Sutton Bonington Campus, University of Nottingham, Loughborough LE12 5RD, United Kingdom. Phone: 44 115 9516119. Fax: 44 115 9516162. E-mail: ian.connerton{at}nottingham.ac.uk.

Applied and Environmental Microbiology, July 2004, p. 3877-3883, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.3877-3883.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nather, G., Alter, T., Martin, A., Ellerbroek, L. (2009). Analysis of risk factors for Campylobacter species infection in broiler flocks. Poult. Sci. 88: 1299-1305 [Abstract] [Full Text]  
  • Atterbury, R. J., Van Bergen, M. A. P., Ortiz, F., Lovell, M. A., Harris, J. A., De Boer, A., Wagenaar, J. A., Allen, V. M., Barrow, P. A. (2007). Bacteriophage Therapy To Reduce Salmonella Colonization of Broiler Chickens. Appl. Environ. Microbiol. 73: 4543-4549 [Abstract] [Full Text]  
  • Coward, C., Grant, A. J., Swift, C., Philp, J., Towler, R., Heydarian, M., Frost, J. A., Maskell, D. J. (2006). Phase-Variable Surface Structures Are Required for Infection of Campylobacter jejuni by Bacteriophages.. Appl. Environ. Microbiol. 72: 4638-4647 [Abstract] [Full Text]  
  • Loc Carrillo, C., Atterbury, R. J., El-Shibiny, A., Connerton, P. L., Dillon, E., Scott, A., Connerton, I. F. (2005). Bacteriophage Therapy To Reduce Campylobacter jejuni Colonization of Broiler Chickens. Appl. Environ. Microbiol. 71: 6554-6563 [Abstract] [Full Text]  
  • Atterbury, R. J., Dillon, E., Swift, C., Connerton, P. L., Frost, J. A., Dodd, C. E. R., Rees, C. E. D., Connerton, I. F. (2005). Correlation of Campylobacter Bacteriophage with Reduced Presence of Hosts in Broiler Chicken Ceca. Appl. Environ. Microbiol. 71: 4885-4887 [Abstract] [Full Text]  
  • El-Shibiny, A., Connerton, P. L., Connerton, I. F. (2005). Enumeration and Diversity of Campylobacters and Bacteriophages Isolated during the Rearing Cycles of Free-Range and Organic Chickens. Appl. Environ. Microbiol. 71: 1259-1266 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»