This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Foster, J. S.
Right arrow Articles by Kolenbrander, P. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Foster, J. S.
Right arrow Articles by Kolenbrander, P. E.
Agricola
Right arrow Articles by Foster, J. S.
Right arrow Articles by Kolenbrander, P. E.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, July 2004, p. 4340-4348, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4340-4348.2004

Development of a Multispecies Oral Bacterial Community in a Saliva-Conditioned Flow Cell

Jamie S. Foster and Paul E. Kolenbrander*

Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland 20892

Received 9 December 2003/ Accepted 2 April 2004

Microbial communities within the human oral cavity are dynamic associations of more than 500 bacterial species that form biofilms on the soft and hard tissues of the mouth. Understanding the development and spatial organization of oral biofilms has been facilitated by the use of in vitro models. We used a saliva-conditioned flow cell, with saliva as the sole nutritional source, as a model to examine the development of multispecies biofilm communities from an inoculum containing the coaggregation partners Streptococcus gordonii, Actinomyces naeslundii, Veillonella atypica, and Fusobacterium nucleatum. Biofilms inoculated with individual species in a sequential order were compared with biofilms inoculated with coaggregates of the four species. Our results indicated that flow cells inoculated sequentially produced biofilms with larger biovolumes compared to those biofilms inoculated with coaggregates. Individual-species biovolumes within the four-species communities also differed between the two modes of inoculation. Fluorescence in situ hybridization with genus- and species-specific probes revealed that the majority of cells in both sequentially and coaggregate-inoculated biofilms were S. gordonii, regardless of the inoculation order. However, the representation of A. naeslundii and V. atypica was significantly higher in biofilms inoculated with coaggregates compared to sequentially inoculated biofilms. Thus, these results indicate that the development of multispecies biofilm communities is influenced by coaggregations preformed in planktonic phase. Coaggregating bacteria such as certain streptococci are especially adapted to primary colonization of saliva-conditioned surfaces independent of the mode of inoculation and order of addition in the multispecies inoculum. Preformed coaggregations favor other bacterial strains and may facilitate symbiotic relationships.

* Corresponding author. Mailing address: National Institutes of Health/NIDCR, Building 30, Room 310, 30 Convent Dr., MSC 4350, Bethesda, MD 20892-4350. Phone: (301) 496-1497. Fax: (301) 402-0396. E-mail: pkolenbrander{at}dir.nidcr.nih.gov.

Applied and Environmental Microbiology, July 2004, p. 4340-4348, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4340-4348.2004




This article has been cited by other articles:

  • Al-Ahmad, A., Follo, M., Selzer, A.-C., Hellwig, E., Hannig, M., Hannig, C. (2009). Bacterial colonization of enamel in situ investigated using fluorescence in situ hybridization. J Med Microbiol 58: 1359-1366 [Abstract] [Full Text]  
  • Periasamy, S., Chalmers, N. I., Du-Thumm, L., Kolenbrander, P. E. (2009). Fusobacterium nucleatum ATCC 10953 Requires Actinomyces naeslundii ATCC 43146 for Growth on Saliva in a Three-Species Community That Includes Streptococcus oralis 34. Appl. Environ. Microbiol. 75: 3250-3257 [Abstract] [Full Text]  
  • Ledder, R. G., Madhwani, T., Sreenivasan, P. K., De Vizio, W., McBain, A. J. (2009). An in vitro evaluation of hydrolytic enzymes as dental plaque control agents. J Med Microbiol 58: 482-491 [Abstract] [Full Text]  
  • van der Mei, H.C., Rustema-Abbing, M., Bruinsma, G.M., Gottenbos, B., Busscher, H.J. (2007). Sequence of Oral Bacterial Co-adhesion and Non-contact Brushing. JDR 86: 421-425 [Abstract] [Full Text]  
  • Al-Ahmad, A., Wunder, A., Auschill, T. M., Follo, M., Braun, G., Hellwig, E., Arweiler, N. B. (2007). The in vivo dynamics of Streptococcus spp., Actinomyces naeslundii, Fusobacterium nucleatum and Veillonella spp. in dental plaque biofilm as analysed by five-colour multiplex fluorescence in situ hybridization. J Med Microbiol 56: 681-687 [Abstract] [Full Text]  
  • Davey, M. E., Duncan, M. J. (2006). Enhanced Biofilm Formation and Loss of Capsule Synthesis: Deletion of a Putative Glycosyltransferase in Porphyromonas gingivalis.. J. Bacteriol. 188: 5510-5523 [Abstract] [Full Text]  
  • Lembke, C., Podbielski, A., Hidalgo-Grass, C., Jonas, L., Hanski, E., Kreikemeyer, B. (2006). Characterization of Biofilm Formation by Clinically Relevant Serotypes of Group A Streptococci. Appl. Environ. Microbiol. 72: 2864-2875 [Abstract] [Full Text]  
  • Kato, S., Haruta, S., Cui, Z. J., Ishii, M., Igarashi, Y. (2005). Stable Coexistence of Five Bacterial Strains as a Cellulose-Degrading Community. Appl. Environ. Microbiol. 71: 7099-7106 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»