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Applied and Environmental Microbiology, July 2004, p. 4363-4366, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4363-4366.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Liquid Serial Dilution Is Inferior to Solid Media for Isolation of Cultures Representative of the Phylum-Level Diversity of Soil Bacteria

Liesbeth Schoenborn,1 Penelope S. Yates,1 Bronwyn E. Grinton,1 Philip Hugenholtz,2 and Peter H. Janssen1*

Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia,1 Division of Ecosystem Sciences, Department of Environmental Science, Policy and Management, University of California, Berkeley, Berkeley, California 94720-31102

Received 7 December 2003/ Accepted 17 March 2004

ABSTRACT

Representatives of only four well-characterized bacterial phyla were isolated from a pasture soil by using liquid serial dilution culture. In contrast, members of Acidobacteria, Verrucomicrobia, and Gemmatimonadetes and of other poorly represented bacterial lineages were isolated in earlier experiments with solidified versions of the same media. We conclude that, contrary to expectation, liquid serial dilution culture is inferior to culturing on solid media for isolating representatives of many bacterial phyla from soil.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3010, Australia. Phone: 61 (3) 8344 5706. Fax: 61 (3) 9347 1540. E-mail: pjanssen{at}unimelb.edu.au.

Applied and Environmental Microbiology, July 2004, p. 4363-4366, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4363-4366.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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