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Applied and Environmental Microbiology, July 2004, p. 4379-4383, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4379-4383.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Validation of a PCR-Based Method for Detection of Food-Borne Thermotolerant Campylobacters in a Multicenter Collaborative Trial

M. H. Josefsen,1 N. Cook,2 M. D'Agostino,2 F. Hansen,3 M. Wagner,4 K. Demnerova,5 A. E. Heuvelink,6 P. T. Tassios,7 H. Lindmark,8 V. Kmet,9 M. Barbanera,10 P. Fach,11 S. Loncarevic,12 and J. Hoorfar1*

Danish Institute for Food and Veterinary Research (DFVF), DK-1790 Copenhagen V,1 Danish Meat Research Institute, 4000 Roskilde, Denmark,3 Defra Central Science Laboratory, Sand Hutton, YO41 1LZ York, United Kingdom,2 Institute for Milk Hygiene and Milk Technology, 1210 Vienna, Austria,4 Institute of Chemical Technology, 16628 Prague 6, Czech Republic,5 Inspectorate for Health Protection and Veterinary Public Health, 7200 AE Zutphen, The Netherlands,6 Department of Microbiology, Medical School, Athens University, 11527 Athens, Greece,7 National Food Agency, 751 26 Uppsala, Sweden,8 Slovak Academy of Science, 4001 Kosice, Slovakia,9 Laboratorio Coop Italia, 40033 Casalecchio, Di Reno Bologna, Italy,10 Agence Française de Sécurité Sanitaire des Aliments, 94700 Maisons Alfort, France,11 National Veterinary Institute, 0033 Oslo, Norway,12

Received 24 November 2003/ Accepted 5 April 2004

ABSTRACT

A PCR-based method for rapid detection of food-borne thermotolerant campylobacters was evaluated through a collaborative trial with 12 laboratories testing spiked carcass rinse samples. The method showed an interlaboratory diagnostic sensitivity of 96.7% and a diagnostic specificity of 100% for chicken samples, while these values were 94.2 and 83.3%, respectively, for pig samples.

* Corresponding author. Mailing address: Danish Institute for Food and Veterinary Research (DFVF), 27 Bülowsvej, DK-1790 Copenhagen V, Denmark. Phone: 45-72 34 62 51. Fax: 45-72 34 60 01. E-mail: jho{at}dfvf.dk.

Applied and Environmental Microbiology, July 2004, p. 4379-4383, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4379-4383.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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