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Applied and Environmental Microbiology, July 2004, p. 4402-4407, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4402-4407.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Arthrobacter aurescens TC1 Atrazine Catabolism Genes trzN, atzB, and atzC Are Linked on a 160-Kilobase Region and Are Functional in Escherichia coli

Kannika Sajjaphan,1,2 Nir Shapir,2,3 Lawrence P. Wackett,2,3,4 Michael Palmer,5 Barbara Blackmon,5 Jeff Tomkins,5 and Michael J. Sadowsky1,2,3*

Department of Soil, Water, and Climate,1 Department of Biochemistry, Molecular Biology, and Biophysics,3 BioTechnology Institute,4 Center for Microbial and Plant Genomics, University of Minnesota, St. Paul, Minnesota 55108,2 Clemson University Genome Institute, Clemson, South Carolina 296345

Received 6 February 2004/ Accepted 24 March 2004

ABSTRACT

Arthrobacter aurescens strain TC1 metabolizes atrazine to cyanuric acid via TrzN, AtzB, and AtzC. The complete sequence of a 160-kb bacterial artificial chromosome clone indicated that trzN, atzB, and atzC are linked on the A. aurescens genome. TrzN, AtzB, and AtzC were shown to be functional in Escherichia coli. Hybridization studies localized trzN, atzB, and atzC to a 380-kb plasmid in A. aurescens strain TC1.

* Corresponding author. Mailing address: Department of Soil, Water, and Climate, 439 Borlaug Hall, 1991 Upper Buford Cir., University of Minnesota, St. Paul, MN 55108. Phone: (612) 624-2706. Fax: (612) 625-2208. E-mail: sadowsky{at}umn.edu.

Applied and Environmental Microbiology, July 2004, p. 4402-4407, Vol. 70, No. 7
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.7.4402-4407.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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