This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Repka, S.
Right arrow Articles by Sivonen, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Repka, S.
Right arrow Articles by Sivonen, K.
Agricola
Right arrow Articles by Repka, S.
Right arrow Articles by Sivonen, K.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 2004, p. 4551-4560, Vol. 70, No. 8
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.8.4551-4560.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Effects of Phosphate and Light on Growth of and Bioactive Peptide Production by the Cyanobacterium Anabaena Strain 90 and Its Anabaenopeptilide Mutant

Sari Repka,{dagger} Minna Koivula,{ddagger} Vesa Harjunpä, Leo Rouhiainen, and Kaarina Sivonen*

Department of Applied Chemistry and Microbiology, University of Helsinki, Helsinki, Finland

Received 2 February 2004/ Accepted 15 April 2004

Cyanobacteria synthesize several types of bioactive secondary metabolites. Anabaena strain 90 produces three types of bioactive peptides, microcystins (inhibitors of protein phosphatases 1 and 2A), anabaenopeptilides, and anabaenopeptins (serine protease inhibitors). To investigate the role of the anabaenopeptilides in Anabaena, wild-type strain 90 (WT) and its anabaenopeptilide deficient mutant (MU) were cultured with various light and phosphate levels to evaluate the effects and coeffects of these growth factors on the concentrations of the three classes of peptides and the growth characteristics. WT and MU grew in comparable ways under the different growth conditions. The total peptide concentration in WT was significantly higher than that in MU (2.5 and 1.4 µg/mg [dry weight], respectively). Interestingly, the average concentration of anabaenopeptins was significantly higher in MU than in WT (0.59 and 0.24 µg/mg [dry weight], respectively). The concentration of microcystins was slightly but not statistically significantly higher in MU than in WT (1.0 and 0.86 µg/mg [dry weight], respectively). In WT, the highest peptide concentrations were usually found after 13 days in cultures grown at medium light intensities (23 µmol m–2 s–1) and with the highest phosphate concentrations (2,600 µg liter–1). In MU, the highest peptide concentrations were found in 13-day-old cultures grown at medium light intensities (23 µmol m–2 s–1) and with phosphate concentrations greater than 100 µg liter–1. The higher concentrations of anabaenopeptins in MU may compensate for the absence of anabaenopeptilides. These findings clearly indicate that these compounds may have some linked function in the producer organism, the nature of which remains to be discovered.

* Corresponding author. Mailing address: Department of Applied Chemistry and Microbiology, Viikki Biocenter, P.O. Box 56, FIN-00014 University of Helsinki, Finland. Phone: 358 9 19159270. Fax: 358 9 19159322. E-mail: Kaarina.Sivonen{at}Helsinki.Fi.

{dagger} Present address: Department of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland.

{ddagger} Present address: Department of Agricultural Engineering and Household Technology, University of Helsinki, Helsinki, Finland.

Applied and Environmental Microbiology, August 2004, p. 4551-4560, Vol. 70, No. 8
0099-2240/04/$08.00+0     DOI: 10.1128/AEM.70.8.4551-4560.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sedmak, B., Carmeli, S., Pompe-Novak, M., Tusek-Znidaric, M., Grach-Pogrebinsky, O., Elersek, T., Zuzek, M. C., Bubik, A., Frangez, R. (2009). Cyanobacterial cytoskeleton immunostaining: the detection of cyanobacterial cell lysis induced by planktopeptin BL1125. J PLANKTON RES 31: 1321-1330 [Abstract] [Full Text]  
  • Briand, E., Gugger, M., Francois, J.-C., Bernard, C., Humbert, J.-F., Quiblier, C. (2008). Temporal Variations in the Dynamics of Potentially Microcystin-Producing Strains in a Bloom-Forming Planktothrix agardhii (Cyanobacterium) Population. Appl. Environ. Microbiol. 74: 3839-3848 [Abstract] [Full Text]  
  • Halstvedt, C. B., Rohrlack, T., Ptacnik, R., Edvardsen, B. (2008). On the effect of abiotic environmental factors on production of bioactive oligopeptides in field populations of Planktothrix spp. (Cyanobacteria). J PLANKTON RES 30: 607-617 [Abstract] [Full Text]  
  • Jahnichen, S., Ihle, T., Petzoldt, T., Benndorf, J. (2007). Impact of Inorganic Carbon Availability on Microcystin Production by Microcystis aeruginosa PCC 7806. Appl. Environ. Microbiol. 73: 6994-7002 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»